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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,248 results for: crispr

Showing: 3851 - 3900 of 23248 results

  39. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  40. RFN_EGFP_84

    Plasmid
    #60076
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  41. RFN_EGFP_83

    Plasmid
    #60075
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. RFN_EGFP_82

    Plasmid
    #60074
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. RFN_EGFP_80

    Plasmid
    #60072
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. pSpCas9(BB)-2A-Blast

    Plasmid
    #118055
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118055-plasmid-map-sequence-id-443898

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  45. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

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  46. lenti-RfxCas13d-Blast-BFP

    Plasmid
    #196726
    Purpose
    Sortable and selectable RfxCas13d.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196726-plasmid-map-sequence-id-389737

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  47. pX551-miniCMV-SpCas9

    Plasmid
    #107031
    Purpose
    AAV plasmid expressing Cas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107031-plasmid-map-sequence-id-208545

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  48. CROPSeq-PP7-p65-HSF

    Plasmid
    #196719
    Purpose
    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196719-plasmid-map-sequence-id-388066

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  49. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid
    #214127
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-VPR
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214127-plasmid-map-sequence-id-424240

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  50. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Andrea Németh
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    101039-plasmid-map-sequence-id-190363

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Showing: 3851 - 3900 of 23248 results