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pFCiR2

Plasmid

#167512

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pJ2018

Plasmid

#140665

Purpose

dCas9, LuxR & Csy4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9, LuxR & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

Plasmid

#62348

Purpose

An empty optimized gRNA expression vector

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

none

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDASH-DI-ω1

Plasmid

#242517

Purpose

Donor I vector in DASH and omega1 vector in GoldenBraid/DASH, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DIS-α2

Plasmid

#242520

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-α1

Plasmid

#242515

Purpose

Donor II vector in DASH and alpha1 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-ω2

Plasmid

#242518

Purpose

Donor II vector in DASH and omega2 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCVA-amiR-PDS

Plasmid

#32911

Depositor

Yule Liu

Article

Tang et al Plant Physiol. 2010 Jun;153(2):632-41. E

Insert

amiR-PDS (Nb)

Use

Plant expression

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP702

Plasmid

#45455

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Expression

Bacterial

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP704nano

Plasmid

#206190

Purpose

Near-infrared fluorescent protein miRFP704nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E1

Plasmid

#15787

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6

Plasmid

#20277

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
mCherry-FDD

Plasmid

#80629

Purpose

Fluorescent destablizing domain for regulating protein stability and fluorescence

Depositor

Thomas Wandless

Article

Navarro et al ACS Chem Biol. 2016 Jun 6.

Insert

UnaG

Use

Retroviral

Tags

HA-mcherry-linker

Mutation

mutations A36V, R136G

Promoter

LTR

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAG416Gal-GFP

Plasmid

#38309

Depositor

Susan Lindquist

Article

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

EGFP (eGFP )

Expression

Yeast

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pAd5-B2

Plasmid

#122552

Purpose

Block 2 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 3737-10881

Use

Synthetic Biology

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CIM5 (Rpt-1) GFP

Plasmid

#16074

Depositor

Erin Schuman

Article

Bingol et al Nature. 2006 Jun 29. 441(7097):1144-8.

Insert

CIM5 (RPT1 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pBTG2

Plasmid

#149475

Purpose

Broad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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6920 pPROEXHsp104b

Plasmid

#1230

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (HSP104 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only
pDmDERusk

Plasmid

#213702

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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bfloGFPa1

Plasmid

#60749

Purpose

Bacterial expression of Amphioxus GFPa1

Depositor

Dimitri Deheyn

Article

Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

Insert

GFPa1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only