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A*11:02:01

Plasmid

#203282

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:02:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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B*14:02:01:01

Plasmid

#203235

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*14:02:01:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B*50:01:01

Plasmid

#203234

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*50:01:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B*13:02:01

Plasmid

#203233

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*13:02:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
A*74:01:01

Plasmid

#203270

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*74:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B*35:03:01

Plasmid

#203255

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*35:03:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B*40:02:01

Plasmid

#203239

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*40:02:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B*55:02:01

Plasmid

#203240

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*55:02:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPI-UPP2-3xFLAG

Plasmid

#201645

Purpose

Over-expression of 3xFLAG-tagged human UPP2

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UPP2 (UPP2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-RPE_sgRNA1

Plasmid

#201618

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

RPE (RPE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-TYMS_sgRNA1

Plasmid

#201628

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-TYMS_sgRNA2

Plasmid

#201629

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-UCK1_sgRNA1

Plasmid

#201630

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK1 (UCK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-UCK1_sgRNA2

Plasmid

#201631

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK1 (UCK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-UCK2_sgRNA1

Plasmid

#201632

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK2 (UCK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-KIF1aMotor-mScarlet-FKBP

Plasmid

#191336

Purpose

Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

motor domain aa 1-405

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-mScarlet-KIFC1motorN593K

Plasmid

#191335

Purpose

Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-mScarlet-KIFC1N593K (KIFC1 Human)

Use

Tags

Expression

Mammalian

Mutation

motor domain aa 125-673; N593K

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_591

Plasmid

#160653

Purpose

Expresses OLE1 under the control of the yeast Sup35 promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

YGL055W (OLE1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Sup35 promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-DCX-TurboID-V5

Plasmid

#190740

Purpose

Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

doublecortin (DCX Human)

Use

Tags

TurboID and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PHR-GFP-STAT2

Plasmid

#183931

Purpose

Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-STAT2 (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge