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Plasmid#99688PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXSE901BG
Plasmid#91714PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHEE901
Plasmid#91707PurposeCRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygRDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHSE901
Plasmid#91708PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygRDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC-H1-gRNA
Plasmid#61089PurposeCRISPR/Cas9 system gRNA cloningDepositorInsertH1 promoter; gRNA sequences
UseCRISPR; /cas9 grna cloning vectorTagsExpressionMammalianMutationPromoterH1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSE901
Plasmid#91709PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-syn pA
Plasmid#99689PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV489
Plasmid#177697PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXSE901B
Plasmid#91713PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIV490
Plasmid#177698PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRHR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-1X snRP1
Plasmid#99687PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIV492
Plasmid#177700PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV488
Plasmid#177696PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast speciesDepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterTDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
loxP-G418-LoxP-TurboID-Rab11 HR
Plasmid#230026PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cellsDepositorInsertgenomic sequence Rab11 locus (RAB11A Human)
UseCRISPRTagsTurbo-ID, V5ExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-p53DD
Plasmid#176902PurposeIVT template plasmid for making p53DD modRNADepositorInsertp53DD
UseIvt template plasmidTagsExpressionMutationPromoterT7 promoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAT31
Plasmid#204291PurposeFor inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJAT7
Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only