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pEarleyGate 100RMOA mPing

Plasmid

#196137

Purpose

Measures mPing transposition in plants

Depositor

Charles Nathan Hancock

Publication

Renken et al MicroPubl Biol. 2023 May 16;2023:10.1791

Inserts

Pong TPase LA T2A ORF1SC1 ONE
mPing

Use

Binary

Expression

Plant

Mutation

TPase NES sequence mutated L418A, L420A; T2A pept…

Promoter

35S and RPS5a

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Gag-delta-p6-mEGFP

Plasmid

#116890

Purpose

expresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cells

Depositor

Sanford Simon

Publication

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

synthetic Gag missing the p6 domain

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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YCe3736_HC_Amp_ccdB_receiver vector

Plasmid

#100637

Purpose

EMMA toolkit receiver vector

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST-FRT/T0-GFP-BRCA1 1364-1437

Plasmid

#71119

Purpose

Mammalian expression of GFP-BRCA1 1364-1437

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1364-1437 of BRCA1

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pVETL-Cmcs-Flag-Ataxin3Q80-C14A

Plasmid

#22121

Depositor

Henry Paulson

Publication

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C14A

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD1 (OTU, aa 287-437)

Plasmid

#61407

Purpose

Expresses human OTUD1 (OTU domain) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-286 and aa 438-481.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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HA-GLUT5 in pRK5

Plasmid

#154205

Purpose

N-terminal HA-tagged GLUT5 cDNA expression vector for transfections

Depositor

David Sabatini

Publication

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

SLC2A5 (SLC2A5 Human)

Tags

N terminal HA tag

Expression

Mammalian

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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TPI in pCW57_tTA_Blast

Plasmid

#154209

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

Depositor

David Sabatini

Publication

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-UBF1-C1

Plasmid

#134532

Purpose

Expresses YFP-UBF1 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

UBF1 (UBTF Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pALPS gag

Plasmid

#101324

Purpose

Encodes codon optimized NL4-3 gag

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 gag

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-Puro-TDP-43-K263E

Plasmid

#133758

Purpose

Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed lysine 263 to glutamate.

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

Plasmid

#74261

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Publication

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.Y591X truncation

Promoter

CMV

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-eNme2-C-BE4max

Plasmid

#183679

Purpose

Expresses eNme2-C-BE4 in mammalian cells

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rAPOBEC1-neNme2-C-2xUGI

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMVA

Plasmid

#121149

Purpose

This plamid was used for improving the production of DMAPP (IPP) in E.coli

Depositor

Minjuan Xu

Publication

He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

Insert

MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )

Use

Synthetic Biology

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PLX_307 GFP

Plasmid

#184492

Purpose

Lentivirus expression for GFP

Depositor

Todd Golub, Peter Tsvetkov

Publication

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

GFP

Use

Lentiviral

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.23 WT 2014 EBOV + Mucin-Like Domain

Plasmid

#85794

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Publication

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Promoter

CMV IE

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB2 (full-length, aa 1-234)

Plasmid

#61421

Purpose

Expresses human OTUB2 (full-length) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPB-EF1A-Puro-hTBK1-EGFP

Plasmid

#221553

Purpose

Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Publication

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLXIN GFP

Plasmid

#132935

Purpose

MLV-based transfer vector expressing GFP and neomycin resistance protein

Depositor

Jeremy Luban

Publication

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

GFP

Use

Lentiviral, RNAi, and Retroviral

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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