165,567 results

1. pAAV-TRE-NpHR-EYFP

   Plasmid

   #171621

   Purpose

   TRE-NpHR-EYFP reporter

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TRE-NpHR-EYFP
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. hfCas12Max

   Plasmid

   #195336

   Purpose

   vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized hfCas12Max
   

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   N243R, E336R, D892R

   Promoter

   CBh, CMV, hU6

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVone-AAV-PHP.eB-CMV-eGFP

   Plasmid

   #230929

   Purpose

   pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system.

   Depositor

   Qizhao Wang

   Article

   Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-DNMT3A-EGFP

   Plasmid

   #71666

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSWAP_Entry_HB

   Plasmid

   #184868

   Purpose

   pSwap plasmid part with HB homology arms

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTRIPZ Myc2-mouse TTP WT

   Plasmid

   #107000

   Purpose

   Expresses epitope-tagged wild-type murine TTP

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   tristetraprolin (Zfp36 Mouse)
   

   Tags

   Double Myc peptide epitope tag

   Expression

   Mammalian

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

   Plasmid

   #87259

   Purpose

   YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

   Depositor

   David Sibley

   Article

   Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

   Inserts

   YFP-mAID-3HA
   HXGPRT
   

   Use

   Toxoplasma expression

   Tags

   3HA and mAID

   Mutation

   Codon optimized for Toxoplasma gondii expression

   Promoter

   TgDHFR-TS 463 bp and TgTUB1 378 bp

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. nls-mCherry

   Plasmid

   #108881

   Purpose

   Expression of nuclear localized mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   nls-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Expression

   Mammalian

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRXIP TMEM41B-GFP

   Plasmid

   #117414

   Purpose

   Expresses TMEM41B tagged with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   TMEM41B (TMEM41B Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCL1271

    Plasmid

    #217963

    Purpose

    Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.

    Depositor

    Andrew Bowman

    Article

    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

    Insert

    Imp5 (IPO5 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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