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DP6

Plasmid

#140446

Purpose

Untargetted bacterial drift

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1 gIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pNICE-NL2A

Plasmid

#15259

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 2 with insert A (Nlgn2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Plasmid

#179106

Purpose

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam

Plasmid

#16440

Purpose

Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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p45sheLL

Plasmid

#37323

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

HPV45L1+L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

Plasmid

#201032

Purpose

Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4e-Kv

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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PB-U6insert-EF1puro

Plasmid

#104537

Purpose

A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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opto-E-cad GFP

Plasmid

#203327

Purpose

Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

Depositor

Seraphine Wegner

Article

Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

Insert

Photoswitchable - E-cadherin with GFP tag (CDH1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Addition of Aslov2 domain at T133 of human E-cadh…

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-BFP

Plasmid

#188775

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Jonathan Weissman, Marco Jost

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Expression

Mutation

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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