We narrowed to 17,784 results for: por
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Plasmid#86212Purposebeta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoterDepositorUsePonasterone a inducible promoterTagslacZA or lacZBExpressionMammalianMutation39TER in beta Globin in MCS IIPromoterPonasterone A inducibleAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only
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pRetroX-TRE3G_NT-GSDMD_I105N_
Plasmid#201174PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralMutationaa1-276 only, I105NPromoterTRE3GAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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CD8-mCherry-FRB
Plasmid#100738PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-QL
Plasmid#86079PurposeA bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
TagsGSTExpressionBacterialMutationChanged glutamine 67 to leucinePromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti_PGK-Cas9-P2A-HygR
Plasmid#164135PurposeLentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cellsDepositorAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-dsRedM
Plasmid#73592PurposeExpress human Plekhm1 in mammalian cellsDepositorAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-TN
Plasmid#86080PurposeA bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
TagsGSTExpressionBacterialMutationChanged threonine 22 to asparginePromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
Tagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
LUC01 (L/Sec/P)
Plasmid#112144PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
ExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U)Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only