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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 7881 - 7890 of 13284 results

  2. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid
    #181788
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181788-plasmid-map-sequence-id-354819

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  3. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid
    #181790
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181790-plasmid-map-sequence-id-354811

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  4. pGG-NES-TurboID-GFP

    Plasmid
    #209401
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    NES-3XHA-TurboID
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available Since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209401-plasmid-map-sequence-id-418454

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  6. pGFP FAT

    Plasmid
    #50517
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Article
    Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.
    Insert
    protein tyrosine kinase, domain
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    truncated (NT2630-3268)
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 7881 - 7890 of 13284 results