4,180 results

1. GFP-C1-PLCdelta-PH

   Plasmid

   #21179

   Purpose

   encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

   Depositor

   Tobias Meyer

   Article

   Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

   Insert

   PLCdelta (PLCD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PH domain of human PLC delta, amino acids Met1–Il…

   Available Since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-NES-jRGECO1a

   Plasmid

   #61563

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRGECO1a
   

   Tags

   nuclear export signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

   Plasmid

   #188768

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK381 (AAVS1 CMV-OsTIR1F74G)

   Plasmid

   #140536

   Purpose

   AAVS1 CMV-OsTIR1(F74G)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CMV-OsTIR1(F74G)
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-2xFLAG-SREBP-1c

   Plasmid

   #26802

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   SREBP-1c (SREBF1 Human)
   

   Tags

   2XFLAG

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. mito-dendra2

   Plasmid

   #55796

   Purpose

   photo-activatable mitochondrial marker for expression in mammalian cells

   Depositor

   David Chan

   Article

   Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

   Insert

   mito-Dendra2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-UbC-rtTA-Ngn2:2A:EGFP

   Plasmid

   #127288

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

   Insert

   Ngn2:2A:Ascl1 (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and EGFP linked via 2A

   Expression

   Mammalian

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 HA-Sumo2 WT

   Plasmid

   #48967

   Purpose

   Expresses HA SUMO2 Wild Type in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO2 (SUMO2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDS221

   Plasmid

   #34981

   Purpose

   Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb1–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 PRDM16

    Plasmid

    #15503

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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