Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,180 results for: yeast

Clear keywords
Showing: 3901 - 3950 of 10180 results

  1. GTU-ir

    Plasmid
    #81097
    Purpose
    Gene Tagging vector URA/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81097-plasmid-map-sequence-id-154264

    Enlarge

  3. pNB786

    Plasmid
    #60151
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60151-plasmid-map-sequence-id-96314

    Enlarge

  4. pNB682

    Plasmid
    #60143
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    A4V
    Promoter
    MET17pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60143-plasmid-map-sequence-id-96214

    Enlarge

  5. Gal4pBS134-Pleum

    Plasmid
    #60339
    Purpose
    Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Expression
    Yeast
    Available Since
    Feb. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    60339-plasmid-map-sequence-id-96620

    Enlarge

  10. p406GAL1

    Plasmid
    #17437
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17437-plasmid-map-sequence-id-8611

    Enlarge

  11. p406GALL

    Plasmid
    #17434
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALL promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17434-plasmid-map-sequence-id-8608

    Enlarge

  12. p405TEF1

    Plasmid
    #15968
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15968-plasmid-map-sequence-id-8453

    Enlarge

  13. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

    Enlarge

  14. p406MET3

    Plasmid
    #17436
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    MET3 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17436-plasmid-map-sequence-id-8610

    Enlarge

  15. p416-GPD-PRUNE-HA

    Plasmid
    #183945
    Purpose
    Expresses human HA-tagged PRUNE from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PRUNE (PRUNE1 Human)
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183945-plasmid-map-sequence-id-363298

    Enlarge

  16. p405GALS

    Plasmid
    #15965
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405GALS (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALS promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15965-plasmid-map-sequence-id-8450

    Enlarge

  17. p405GALL

    Plasmid
    #15964
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405GALL (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALL promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15964-plasmid-map-sequence-id-8449

    Enlarge

  18. pBCSK_HIS3_II

    Plasmid
    #109059
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    HIS3
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109059-plasmid-map-sequence-id-209551

    Enlarge

  19. pBCSK_HIS3_I

    Plasmid
    #109058
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    HIS3
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109058-plasmid-map-sequence-id-209548

    Enlarge

  20. pTH340-SUP45(1-242)-GalBD

    Plasmid
    #29740
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 1-242 of the yeast SUP45 gene only
    Available Since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29740-plasmid-map-sequence-id-13824

    Enlarge

  21. p404GALL

    Plasmid
    #17418
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALL promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17418-plasmid-map-sequence-id-8593

    Enlarge

  22. Sc Pol1-FLAG-pRS402/GAL-L

    Plasmid
    #241975
    Purpose
    Over-express Sc Pol1-FLAG (Sc pol alpha - large subunit) in yeast (integrated)
    Depositor
    Michael O'Donnell
    Insert
    Pol1-FLAG (POL1 Budding Yeast)
    Tags
    3X FLAG
    Expression
    Yeast
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241975-plasmid-map-sequence-id-483375

    Enlarge

  23. Sc FLAG-S6-RFC1-pRS405/GAL-L

    Plasmid
    #239473
    Purpose
    Overexpress Sc FLAG-S6-RFC1 in yeast (integrated)
    Depositor
    Michael O'Donnell
    Insert
    Sc FLAG-S6-RFC1 (RFC1 Budding Yeast)
    Tags
    3X FLAG followed by S6
    Expression
    Yeast
    Available Since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    239473-plasmid-map-sequence-id-475709

    Enlarge

  25. pML107-KAE1

    Plasmid
    #232891
    Purpose
    Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    KAE1 gRNA (KAE1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232891-plasmid-map-sequence-id-470392

    Enlarge

  26. pML107-SAM50

    Plasmid
    #232895
    Purpose
    Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    SAM50 gRNA (SAM50 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232895-plasmid-map-sequence-id-470389

    Enlarge

  27. pML107-ARB1

    Plasmid
    #232888
    Purpose
    Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ARB1 gRNA (ARB1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232888-plasmid-map-sequence-id-464740

    Enlarge

  28. pML107-RER2

    Plasmid
    #232884
    Purpose
    Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    RER2 gRNA (RER2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232884-plasmid-map-sequence-id-462289

    Enlarge

  29. pML104-LEU2-3

    Plasmid
    #232894
    Purpose
    Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    LEU2 gRNA (RER2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232894-plasmid-map-sequence-id-462276

    Enlarge

  30. pML107-AVO1

    Plasmid
    #232889
    Purpose
    Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    AVO1 gRNA (AVO1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232889-plasmid-map-sequence-id-462277

    Enlarge

  31. pML107-YFR054C

    Plasmid
    #232900
    Purpose
    Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    YFR054C gRNA (YFR054C Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232900-plasmid-map-sequence-id-462143

    Enlarge

  32. pML107-ZIM17

    Plasmid
    #232901
    Purpose
    Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ZIM17 gRNA (ZIM17 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232901-plasmid-map-sequence-id-462145

    Enlarge

  33. pML107-SRP14

    Plasmid
    #232896
    Purpose
    Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    SRP14 gRNA (SRP14 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232896-plasmid-map-sequence-id-462141

    Enlarge

  34. pML107-TLG1

    Plasmid
    #232897
    Purpose
    Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    TLG1 gRNA (TLG1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232897-plasmid-map-sequence-id-462140

    Enlarge

  35. pML107-VHT1

    Plasmid
    #232898
    Purpose
    Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    VHT1 gRNA (VHT1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232898-plasmid-map-sequence-id-462142

    Enlarge

  36. pML107-ADE13

    Plasmid
    #232887
    Purpose
    Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ADE13 gRNA (ADE13 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232887-plasmid-map-sequence-id-462137

    Enlarge

  37. pML107-LAS17

    Plasmid
    #232892
    Purpose
    Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    LAS17 gRNA (LAS17 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232892-plasmid-map-sequence-id-462139

    Enlarge

  38. pML104-LEU2-2

    Plasmid
    #232883
    Purpose
    Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    LEU2 gRNA (LEU2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232883-plasmid-map-sequence-id-462088

    Enlarge

  39. pML107-INO80

    Plasmid
    #232890
    Purpose
    Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    INO80 gRNA (INO80 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232890-plasmid-map-sequence-id-462082

    Enlarge

  41. pRS415 GPDpro-RNQ1-CFP

    Plasmid
    #224887
    Purpose
    Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).
    Depositor
    Paul Herman
    Insert
    RNQ1 (RNQ1 Budding Yeast)
    Tags
    CFP
    Expression
    Yeast
    Promoter
    GPD
    Available Since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224887-plasmid-map-sequence-id-448181

    Enlarge

  42. p416-GPD-HA-PPK(mutant)

    Plasmid
    #183944
    Purpose
    Expresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK(mutant) (ppk E. coli)
    Tags
    HA
    Expression
    Yeast
    Mutation
    mutations in His435, His454, and His592 to Alanine
    Promoter
    GPD
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183944-plasmid-map-sequence-id-363303

    Enlarge

  43. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166091-plasmid-map-sequence-id-329609

    Enlarge

  44. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

    Enlarge

  45. pNSU299_RRT8

    Plasmid
    #166080
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RRT8 (RRT8 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166080-plasmid-map-sequence-id-330356

    Enlarge

  46. pNSU299_CPR1_2

    Plasmid
    #166071
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic region near CPR1
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166071-plasmid-map-sequence-id-330355

    Enlarge

  47. pNSU299_GAL7

    Plasmid
    #166089
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL7 (GAL7 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166089-plasmid-map-sequence-id-330353

    Enlarge

  48. pNSU299_GLK1

    Plasmid
    #166090
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GLK1 (GLK1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166090-plasmid-map-sequence-id-329612

    Enlarge

  49. pNSU299_LHS1

    Plasmid
    #166092
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LHS1 (LHS1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166092-plasmid-map-sequence-id-329611

    Enlarge

  50. pNSU299_RPL22A

    Plasmid
    #166079
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL22A (RPL22A Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166079-plasmid-map-sequence-id-329605

    Enlarge

Showing: 3901 - 3950 of 10180 results