We narrowed to 3,642 results for: XIA

1. HA-HIF1alpha P402A/P564A-pBabe-puro

   Plasmid

   #19005

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 402 to Alanin…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

   Plasmid

   #44027

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P405A, P530V, N851A

   Promoter

   CMV

   Available since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-3xFLAG-SRSF1 siRNA-resitant

   Plasmid

   #218974

   Purpose

   Vector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   SRSF1 cDNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…

   Promoter

   CMV

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiU6-LacZ-GFP-Puro (BB)

   Plasmid

   #170459

   Purpose

   For cloning gRNA block

   Depositor

   Yonglun Luo

   Article

   Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

   Insert

   hU6-BB-LacZ and EF1A-EGFP-2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 for gRNA and EF1A for EGFP-2A-Puro

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCKOv3

   Plasmid

   #209046

   Purpose

   Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3b(1454/3172)P2P-mut

   Plasmid

   #21405

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   hypoxia response element mutated

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pDONR223-TAF5-FL_WobbleMut

   Plasmid

   #209053

   Purpose

   Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 full length
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-TAF5-∆ex8_WobbleMut

   Plasmid

   #209054

   Purpose

   Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGLS3-5xHRE-p53(K164R)

   Plasmid

   #72555

   Purpose

   Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

   Depositor

   Ester Hammond

   Article

   Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   5XHRE

   Expression

   Mammalian

   Mutation

   K164R

   Promoter

   E1B minimal promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET01-TAF5-ex8-Minigene-SRSF1 motif WT

    Plasmid

    #218972

    Purpose

    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV LTR

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ppyCAG_RNaseH1_WKKD

    Plasmid

    #111905

    Purpose

    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

    Promoter

    Available since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

    Plasmid

    #44028

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P402A, P577A, N813A

    Promoter

    CMV

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGLS3-5xHRE-p53(K120R)

    Plasmid

    #72554

    Purpose

    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    K120R

    Promoter

    E1B minimal promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-opCas12a-6xNLS

    Plasmid

    #209022

    Purpose

    Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-UltraCas12a-6xNLS

    Plasmid

    #209023

    Purpose

    Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    UltraCas12a-6xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    M537R/F870L

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-enCas12a-2xNLS

    Plasmid

    #209021

    Purpose

    Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    enCas12a-2xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R/K548R

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-HIF2α P405A/P531A-pBabe-Hygro

    Plasmid

    #21676

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids from wt: Proline 405 t…

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-zeo: alpha6mGFP

    Plasmid

    #35611

    Purpose

    mammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tag

    Depositor

    Henry Lester

    Article

    Xiao et al Biochem Pharmacol. 2011 Oct 15;82(8):852

    Insert

    nAChr alpha6mEGFP (Chrna6 Mouse)
    

    Use

    Tags

    mEGFP inserted after A405 with AGA linker on eith…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-miniTurbo-TAF5-FL

    Plasmid

    #209051

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to full length TAF5 isoform

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 full length
    

    Use

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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