We narrowed to 16,014 results for: grna

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E42_gControl_dTet_IRES_mTurquoise2

Plasmid

#189799

Purpose

Retroviral delivery of control guide RNA

Depositor

Ellen Rothenberg

Publication

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Luciferase gRNA

Use

Retroviral

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-2xGFP KI

Plasmid

#183443

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 2xGFP donor

Use

CRISPR

Expression

Mammalian

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-Halo KI

Plasmid

#183433

Purpose

CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-3xGFP KI

Plasmid

#183444

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 3xGFP donor

Use

CRISPR

Expression

Mammalian

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI

Plasmid

#131485

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor (Grin1, Grin1 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0009

Plasmid

#42249

Purpose

gRNA targeted to zebrafish gene tph1a

Depositor

Keith Joung

Publication

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-tph1a (tph1a Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

Plasmid

#67984

Purpose

Cas9 activity reporter with GFP and BFP

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

Plasmid

#67976

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmAG cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Px461-Cas9n-Trp53-sgRNA-alpha

Plasmid

#88846

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Publication

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
px458_2A_GFP_sgRNA_PELO

Plasmid

#127124

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA PELO (PELO Human)

Use

CRISPR

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNf2#1/Cre

Plasmid

#173643

Purpose

Expresses a Nf2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Publication

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf2 (Nf2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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CasRx gRNA targeting EZH2

Plasmid

#219655

Purpose

hU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.

Depositor

Weixin Tang

Publication

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CasRx gRNA targeting EZH2

Use

CRISPR

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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AM77_pU6.Sa-gRNA.CLYBL

Plasmid

#199237

Purpose

Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Publication

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

S. aureus gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C6

Plasmid

#160457

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Publication

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_hPAX7-Promoter_C6

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C5

Plasmid

#160456

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Publication

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_hPAX7-Promoter_C5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C4

Plasmid

#160455

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Publication

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_hPAX7-Promoter_C4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C3

Plasmid

#160454

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Publication

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_hPAX7-Promoter_C3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ173

Plasmid

#98410

Purpose

Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)

Use

CRISPR

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB11785

Plasmid

#212699

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Publication

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Expression

Yeast

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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