Addgene

We narrowed to 3,518 results for: mak

Showing: 1561 - 1580 of 3518 results

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pRRL_U2AF1_WT_mCherry

Plasmid

#84017

Purpose

expression of U2AF1 in mammalian cells

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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FusionRed-pBAD

Plasmid

#54677

Purpose

Localization: Protein Expression Vector , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Type

Empty backbone

Use

Tags

6xHis and FusionRed

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCAPB02

Plasmid

#65628

Purpose

A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis

Depositor

Pei-yuan Qian

Article

Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c1

Plasmid

#120872

Purpose

Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12g1

Plasmid

#120879

Purpose

Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12g1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA-Cdk9

Plasmid

#28102

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Use

Tags

3x HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST_sgRNA

Plasmid

#127921

Purpose

Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)

Use

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c2

Plasmid

#120873

Purpose

Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAJT194

Plasmid

#202293

Purpose

Type IIS cloning plasmid for LPattB5 integrations w/ OriT

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FusionRed-Lifeact-7

Plasmid

#54778

Purpose

Localization: Actin, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Type

Empty backbone

Use

Tags

FusionRed-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMRX-IP-3xFLAG-NCOA4 (dIDR2)

Plasmid

#192811

Purpose

Stable expression of 3xFLAH-NCOA4 (delta IDR2) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the IDR2

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAJT195

Plasmid

#202294

Purpose

Type IIS cloning plasmid for LPattB7 integrations w/ OriT

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dN)

Plasmid

#192808

Purpose

Stable expression of 3xFLAH-NCOA4 (delta N) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the N-terminal domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dM)

Plasmid

#192810

Purpose

Stable expression of 3xFLAH-NCOA4 (delta M) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the Middle domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dIDR1)

Plasmid

#192809

Purpose

Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the IDR1

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dC)

Plasmid

#192812

Purpose

Stable expression of 3xFLAH-NCOA4 (delta C) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the C-terminal domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (I56E)

Plasmid

#192813

Purpose

Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

I56E mutation in the N-terminal domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (L63R)

Plasmid

#192814

Purpose

Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

L63R mutation in the N-terminal domain

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pKM427

Plasmid

#210788

Purpose

L5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transfer

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2021;2314:301-321. doi

Insert

Defective hygromycin resistant gene

Use

Tags

Expression

Bacterial

Mutation

Changed hygromycin resistant gene W190 codon (TGG…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLG027 (qatABCD)

Plasmid

#157905

Purpose

Expresses the qatABCD defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

qatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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