We narrowed to 1,667 results for: plasmid for e coli

1. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Use

   Tags

   3x-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. strep-IRF8

   Plasmid

   #102872

   Purpose

   Express strep-tagged mouse IRF8 protein in E. coli

   Depositor

   Gary Stormo

   Article

   Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

   Insert

   IRF8 (Irf8 Mouse)
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ALFY-FYVE 1G

   Plasmid

   #197045

   Purpose

   Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

   Insert

   ALFY (WDFY3 Human)
   

   Use

   Tags

   6xHis-GST-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ALIX(P801G)-Strep

   Plasmid

   #180024

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX(1-868, P801G)-Strep (PDCD6IP Human)
   

   Use

   Tags

   Strep tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   P801G

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXD71Cas10.1-Pct5.1-crRNA(dadR)

   Plasmid

   #191634

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPC027.306

   Plasmid

   #171149

   Purpose

   Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J306 scRNA
   J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J3-BBa_J23117

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLei-sfGFP-D134*

   Plasmid

   #191110

   Purpose

   An E. coli sfGFP reporter with one TAG at position 134

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing D134 to TAG

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLei-sfGFP-V2*D134*

   Plasmid

   #191111

   Purpose

   An E. coli sfGFP reporter with two TAG at positions 2 & 134

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing V2 & D134 to TAG

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-VHH-ddFLN4-HIS-ybbR

   Plasmid

   #169137

   Purpose

   E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   VHH
   

   Use

   Tags

   6x His tag, ddFLN4 tag, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)

    Plasmid

    #191641

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔDBD

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)

    Plasmid

    #191642

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔTM

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)

    Plasmid

    #191643

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Linker region sequence shuffled

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid

    #182305

    Purpose

    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    

    Use

    Tags

    A modified TorA periplasmic targeting sequence pl…

    Expression

    Bacterial

    Mutation

    Spontaneous mutation of calmodulin's penulti…

    Promoter

    t7

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P1-optimizedNedd4.2F823D

    Plasmid

    #165105

    Purpose

    bacterial expression of a GST fusion of Human Nedd4.2d F823D

    Depositor

    Arthur Haas

    Article

    Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

    Insert

    Nedd4.2d F823D (NEDD4L Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression; F823D; …

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK174

    Plasmid

    #71835

    Purpose

    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7 and endogenous

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK175

    Plasmid

    #71132

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMCherry F8-2

    Plasmid

    #186034

    Purpose

    Express VHH F8-2 as a mCherry fusion in mammalian cells

    Depositor

    Isabelle Landrieu

    Article

    Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

    Insert

    VHH F8-2
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p164-pAAV-mDlx-FLEX-mScarlet

    Plasmid

    #204689

    Purpose

    Fluorescent reporter under the control of the Dlx promotor

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    mScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    mScarlet palmitoylation

    Expression

    Mammalian

    Mutation

    Promoter

    mDlx

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234441

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-syn-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234437

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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