165,652 results

1. pdCas9-DNMT3A-EGFP

   Plasmid

   #71666

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRG203MX

   Plasmid

   #64521

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2-SynTetOff-palGFP

   Plasmid

   #190232

   Purpose

   AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   palmitoylation signal sequence

   Expression

   Mammalian

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3 GCN1-3xFlag_IRES-iRFP

   Plasmid

   #198388

   Purpose

   GCN1 expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   GCN1 (GCN1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. sFlt1-i13 V5

   Plasmid

   #86044

   Purpose

   human Soluble Flt1 isoform i13 (terminates in intron 13)

   Depositor

   Christie Thomas

   Article

   Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

   Insert

   human soluble Flt1 isoform i13 (FLT1 Human)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   isoform i13 (terminates in intron 13)

   Promoter

   CMV

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE HA Chk2 WT

   Plasmid

   #41901

   Depositor

   Stephen Elledge

   Article

   Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

   Insert

   CHK2 (CHEK2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 mTSP1

   Plasmid

   #12405

   Depositor

   Paul Bornstein

   Insert

   TSP1 (Thbs1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

   Plasmid

   #228663

   Purpose

   Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Zika virus portion of NS2B protease coexpressed with NS3 protease
   

   Tags

   His-GST-TEV

   Expression

   Bacterial

   Available Since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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