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Plasmid#67670PurposeGateway entry clone containing mKate2-P2A-APEX2 with no stop codonDepositorInsertmKate2-P2A-APEX2-NS
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
AKT-APEX2-P2A-mKate2
Plasmid#67654PurposeMammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertAKT-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-APEX2
Plasmid#117751PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
BTK-APEX2-P2A-mKate2
Plasmid#67653PurposeMammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertBTK-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST(frt)(ATG_V5-APEX2_noSTOP)
Plasmid#168207PurposeDestination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selectionDepositorTypeEmpty backboneUseUnspecified; Pdest gatewayTagsV5 APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-2xML1N(7Q)
Plasmid#67666PurposeMammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-ML1Nmut*2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
MmCavin4-APEX2-P2A-mKate2
Plasmid#170524PurposeVector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJFDepositorInsertCavin4 (Cavin4 Mouse)
UseTagsAPEX2-P2A-mKate2ExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-LAMP1-EGFP-APEX2-V5
Plasmid#236402PurposeExpresses a fluorescent lysosome-localized APEX2DepositorInsertLAMP1-EGFP-APEX2-V5
UseRetroviralTagsAPEX2, EGFP, and V5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-EGFP-APEX2-V5-EEA1
Plasmid#236403PurposeExpresses a fluorescent endosome-localized APEX2DepositorInsertEGFP-APEX2-V5-EEA1
UseRetroviralTagsAPEX2, EGFP, and V5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Pex3-EGFP-APEX2-V5
Plasmid#236401PurposeExpresses a fluorescent peroxisome-localized APEX2DepositorInsertPex3(fragment)-EGFP-APEX2-V5
UseRetroviralTagsAPEX2, EGFP, and V5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-EGFP-ADRP-APEX2-V5
Plasmid#236405PurposeExpresses a fluorescent lipid droplet-localized APEX2DepositorInsertEGFP-ADRP-APEX2-V5
UseRetroviralTagsAPEX2, EGFP, and V5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW-RUSH-LAMP1-V5-APEX2
Plasmid#228524PurposeLentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsStreptavidin Binding Protein (SBP), Streptavidin-…ExpressionMammalianMutationPromoterUbcAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
OMM-Flag-APEX2-SOPP3
Plasmid#229073PurposeExpresses APEX2-SOPP3 targeted to outer mitochondrial membraneDepositorInsertExpress APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX2-SOPP3-3xNLS
Plasmid#229074Purposeexpresses APEX2-SOPP3 in the mammalian nucleusDepositorInsertExpress APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
COX8mls-Flag-APEX2-SOPP3
Plasmid#229075PurposeExpresses APEX2-SOPP3 in the mitochondrial matrixDepositorInsertExpress APEX2-SOPP3 in the mitochondrial matrix (APEX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-SRRD-V5-APEX2 (in pLEX_307)
Plasmid#214921Purposeconstitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylationDepositorInsertSRRD (SRRD Human)
UseLentiviralTagsV5-APEX2ExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKD6-V5-APEX2-NES
Plasmid#178700PurposeAAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)DepositorInsertV5-APEX2-NES
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS2-V5-APEX2-NES
Plasmid#178702PurposeAAV vector for Cre-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)DepositorInsertV5-APEX2-NES
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS3-V5-APEX2-NES
Plasmid#178703PurposeAAV vector for Flp-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)DepositorInsertV5-APEX2-NES
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS4-V5-APEX2-NES
Plasmid#178704PurposeAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)DepositorInsertV5-APEX2-NES
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only