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We narrowed to 1,740 results for: plasmid for e coli

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Showing: 351 - 400 of 1740 results

  2. pSP019

    Plasmid
    #168195
    Purpose
    expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.
    Depositor
    James Fleckenstein
    Insert
    eatA[H134R]
    Expression
    Bacterial
    Mutation
    H134R mutation
    Promoter
    araBAD
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    168195-plasmid-map-sequence-id-339468

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  6. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. FruR-pHG165a (CRA)

    Plasmid
    #90066
    Purpose
    full length WT FruR (CRA)
    Depositor
    Liskin Swint-Kruse
    Insert
    FruR (CRA)
    Expression
    Bacterial
    Promoter
    Iq
    Available Since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    90066-plasmid-map-sequence-id-178401

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  17. pORTMAGE502B

    Plasmid
    #128971
    Purpose
    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    xyl-Pm
    Available Since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    128971-plasmid-map-sequence-id-252654

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  18. pORTMAGE302A

    Plasmid
    #128970
    Purpose
    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    cI-pL
    Available Since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128970-plasmid-map-sequence-id-252505

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  19. His6-TEV-LplA W37V and C55M

    Plasmid
    #248819
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and C55M
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248819-plasmid-map-sequence-id-495682

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  20. His6-TEV-LplA W37V and P167I

    Plasmid
    #248816
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and P167I
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248816-plasmid-map-sequence-id-495681

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  21. His6-TEV-LplA W37V and A48R

    Plasmid
    #248821
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and A48R
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248821-plasmid-map-sequence-id-495679

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  22. His6-TEV-LplA W37V and T57I

    Plasmid
    #248818
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and T57I
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248818-plasmid-map-sequence-id-495671

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  23. His6-TEV-LplA W37V and F147S

    Plasmid
    #248820
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and F147S
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248820-plasmid-map-sequence-id-495669

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  24. His6-TEV-LplA W37V and E265R

    Plasmid
    #248824
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and E265R
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248824-plasmid-map-sequence-id-495668

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  25. His6-TEV-LplA W37V and Y139S

    Plasmid
    #248822
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and Y139S
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248822-plasmid-map-sequence-id-495666

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  26. His6-TEV-LplA W37V and A48N

    Plasmid
    #248823
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and A48N
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248823-plasmid-map-sequence-id-495661

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  27. His6-TEV-LplA W37V and L172A

    Plasmid
    #248815
    Purpose
    Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    W37V and L172A
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248815-plasmid-map-sequence-id-495659

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  28. pLNKR1

    Plasmid
    #67968
    Purpose
    High copy number plasmid encoding part of the tra Operon PY-traR
    Depositor
    Joshua Leonard
    Insert
    tra Operon (First Quarter)
    Use
    Synthetic Biology
    Available Since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67968-plasmid-map-sequence-id-121285

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  29. pLNKR2

    Plasmid
    #67969
    Purpose
    High copy number plasmid encoding part of the tra Operon traC-artA
    Depositor
    Joshua Leonard
    Insert
    tra Operon (second Quarter)
    Use
    Synthetic Biology
    Available Since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67969-plasmid-map-sequence-id-121291

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  30. pBad-HPII

    Plasmid
    #105839
    Purpose
    expresses E. coli HPII catalase with N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Tags
    6x his tag
    Expression
    Bacterial
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105839-plasmid-map-sequence-id-205523

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  31. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid
    #127637
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    YPet
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PP7 coat protein (PCP)
    Expression
    Bacterial
    Promoter
    pJ21306
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127637-plasmid-map-sequence-id-251705

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  32. pJaM

    Plasmid
    #67966
    Purpose
    Inducible TraJ, TraM expression with arabinose.
    Depositor
    Joshua Leonard
    Insert
    TraM, TraJ
    Use
    Synthetic Biology
    Promoter
    pBAD
    Available Since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67966-plasmid-map-sequence-id-118466

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  33. pJ

    Plasmid
    #67965
    Purpose
    Indicuble TraJ expression with arabinose.
    Depositor
    Joshua Leonard
    Insert
    TraJ
    Use
    Synthetic Biology
    Tags
    FLAG
    Promoter
    pBAD
    Available Since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67965-plasmid-map-sequence-id-118469

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  35. MBP_TS-B1AR_Trx_PreSc_NdeI_AgeI

    Plasmid
    #247768
    Purpose
    This plasmid is a modified version of pRG/III-hs-MBP (Addgene plasmid #121676), designed for the expression of a functional β1-adrenergic receptor (β1AR) in E. coli.
    Depositor
    Stephan Grzesiek
    Article
    Abiko et al J Biomol NMR. 2021 Jan;75(1):25-38. doi:
    Insert
    Thermostable Turkey B1-adrenergic receptor (TS-B1AR)
    Tags
    MBP–(GS)5–HRV 3C site–(GS)5–NdeI site_ B1AR_AgeI …
    Expression
    Bacterial
    Promoter
    lac promoter
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247768-plasmid-map-sequence-id-494567

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  36. pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

    Plasmid
    #127636
    Purpose
    Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    mTurquoiuse2-Tat, YPet-PCP
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PCP and RNA binding peptide:…
    Expression
    Bacterial
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127636-plasmid-map-sequence-id-251704

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  37. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Expression
    Bacterial
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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  38. His6-mCherry-Flag-LplAW37V and T57I

    Plasmid
    #248806
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and T57I
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248806-plasmid-map-sequence-id-495680

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  39. His6-mCherry-Flag-LplAW37V and P167I

    Plasmid
    #248804
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and P167I
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248804-plasmid-map-sequence-id-495677

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  40. His6-mCherry-Flag-LplAW37V and Y139S

    Plasmid
    #248810
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and Y139S
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248810-plasmid-map-sequence-id-495676

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  41. His6-mCherry-Flag-LplAW37V and A48R

    Plasmid
    #248809
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and A48R
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248809-plasmid-map-sequence-id-495675

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  42. His6-mCherry-Flag-LplAW37V and A48N

    Plasmid
    #248811
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and A48N
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248811-plasmid-map-sequence-id-495674

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  43. His6-mCherry-Flag-LplAW37V and F147S

    Plasmid
    #248808
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and F147S
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248808-plasmid-map-sequence-id-495663

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  44. His6-mCherry-Flag-LplA Wild Type

    Plasmid
    #248801
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248801-plasmid-map-sequence-id-495402

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  45. His6-mCherry-Flag-LplAW37V and L172A

    Plasmid
    #248803
    Purpose
    Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis-mCherry-Flag
    Expression
    Mammalian
    Mutation
    W37V and L172A
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248803-plasmid-map-sequence-id-495399

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  49. pdUA-lacIN

    Plasmid
    #141079
    Purpose
    Plasmid vector for testing DNA binding proteins by the I-Block assay.
    Depositor
    Antal Kiss
    Article
    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28
    Type
    Empty backbone
    Use
    Vector for the i-block assay designed to study dn…
    Expression
    Bacterial
    Promoter
    Escherichia coli lacIN promoter
    Available Since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    141079-plasmid-map-sequence-id-310860

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  50. pSB8

    Plasmid
    #82037
    Purpose
    Hybrid CitA[1-193]-Tar[204-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: CitA[1-193]-Tar[204-553]
    Available Since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82037-plasmid-map-sequence-id-174335

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Showing: 351 - 400 of 1740 results