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We narrowed to 1,640 results for: plasmid for e coli

Showing: 351 - 400 of 1640 results

  1. pHelper_O

    Plasmid
    #173783
    Purpose
    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    186 Integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    E59G - please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173783-plasmid-map-sequence-id-342367

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  4. FruR-pHG165a (CRA)

    Plasmid
    #90066
    Purpose
    full length WT FruR (CRA)
    Depositor
    Liskin Swint-Kruse
    Insert
    FruR (CRA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Iq
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    90066-plasmid-map-sequence-id-178401

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  5. pE_Cre_lambdaCos

    Plasmid
    #173785
    Purpose
    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    Cre
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    S102Y and S279Y- please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173785-plasmid-map-sequence-id-342369

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  6. pORTMAGE502B

    Plasmid
    #128971
    Purpose
    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    xyl-Pm
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    128971-plasmid-map-sequence-id-252654

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  7. pORTMAGE302A

    Plasmid
    #128970
    Purpose
    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    cI-pL
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128970-plasmid-map-sequence-id-252505

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  8. pLNKR1

    Plasmid
    #67968
    Purpose
    High copy number plasmid encoding part of the tra Operon PY-traR
    Depositor
    Joshua Leonard
    Insert
    tra Operon (First Quarter)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67968-plasmid-map-sequence-id-121285

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  9. pLNKR2

    Plasmid
    #67969
    Purpose
    High copy number plasmid encoding part of the tra Operon traC-artA
    Depositor
    Joshua Leonard
    Insert
    tra Operon (second Quarter)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67969-plasmid-map-sequence-id-121291

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  10. pBad-HPII

    Plasmid
    #105839
    Purpose
    expresses E. coli HPII catalase with N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105839-plasmid-map-sequence-id-205523

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  11. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid
    #127637
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    YPet
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PP7 coat protein (PCP)
    Expression
    Bacterial
    Mutation
    Promoter
    pJ21306
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127637-plasmid-map-sequence-id-251705

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  12. pJaM

    Plasmid
    #67966
    Purpose
    Inducible TraJ, TraM expression with arabinose.
    Depositor
    Joshua Leonard
    Insert
    TraM, TraJ
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67966-plasmid-map-sequence-id-118466

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  13. pJ

    Plasmid
    #67965
    Purpose
    Indicuble TraJ expression with arabinose.
    Depositor
    Joshua Leonard
    Insert
    TraJ
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67965-plasmid-map-sequence-id-118469

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  15. pDisCoTune

    Plasmid
    #173713
    Purpose
    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.
    Depositor
    Morten Norholm
    Article
    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2
    Inserts
    ERV1 (ERV1 Budding Yeast)
    PDI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    173713-plasmid-map-sequence-id-342928

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  16. pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

    Plasmid
    #127636
    Purpose
    Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    mTurquoiuse2-Tat, YPet-PCP
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PCP and RNA binding peptide:…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127636-plasmid-map-sequence-id-251704

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  17. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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  18. pLPE1

    Plasmid
    #209981
    Purpose
    Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (pMB1_ampR_B0017)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209981-plasmid-map-sequence-id-419436

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  19. pLPE2

    Plasmid
    #209982
    Purpose
    Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (ampR_B0017)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209982-plasmid-map-sequence-id-419966

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  21. pdUA-lacIN

    Plasmid
    #141079
    Purpose
    Plasmid vector for testing DNA binding proteins by the I-Block assay.
    Depositor
    Antal Kiss
    Article
    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28
    Type
    Empty backbone
    Use
    Vector for the i-block assay designed to study dn…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Escherichia coli lacIN promoter
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    141079-plasmid-map-sequence-id-310860

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  22. pSB8

    Plasmid
    #82037
    Purpose
    Hybrid CitA[1-193]-Tar[204-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: CitA[1-193]-Tar[204-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82037-plasmid-map-sequence-id-174335

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  23. pSB11

    Plasmid
    #82040
    Purpose
    Hybrid NarQ[1-217]-Tar[257-553] expression plasmid
    Depositor
    Victor Sourjik
    Article
    Bi et al ACS Synth Biol. 2016 Jun 10.
    Insert
    Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase NarQ: NarQ[1-217]-Tar[257-553]
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    82040-plasmid-map-sequence-id-174334

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  39. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Opher Gileadi
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Use
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159615-plasmid-map-sequence-id-311066

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  40. pGBW-m4046865

    Plasmid
    #153930
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153930-plasmid-map-sequence-id-307315

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  43. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Use
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  45. pBSJL1

    Plasmid
    #71261
    Purpose
    E. coli-Veillonella shuttle plasmid
    Depositor
    Felicia Qi
    Article
    Liu et al Appl Environ Microbiol. 2012 May;78(9):3
    Type
    Empty backbone
    Use
    E. coli-veillonella shuttle plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  46. pHL1726

    Plasmid
    #53032
    Purpose
    PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and hfqlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. pXU01

    Plasmid
    #212151
    Purpose
    Plasmid expressing xylitol dehydrogenase under inducible promoter PT7 control. Plasmid confers the ability to use xylitol as major carbon source to E. coli.
    Depositor
    Kang Zhou
    Article
    Fung et al Biomaterials. 2022 Aug;287:121661. doi:
    Insert
    xylitol dehydrogenase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212151-plasmid-map-sequence-id-420771

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  48. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

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  49. pBad-HPII TAG392

    Plasmid
    #105848
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 392 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 392
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105848-plasmid-map-sequence-id-205565

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  50. pBad-HPII TAG415

    Plasmid
    #105847
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 415 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 415
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105847-plasmid-map-sequence-id-205555

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Showing: 351 - 400 of 1640 results