We narrowed to 152 results for: AsCas12a

1. pRDA_886

   Plasmid

   #201162

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_887

   Plasmid

   #201164

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRDA_888

   Plasmid

   #201165

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. enAsABE8e-dimer

   Plasmid

   #138507

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ223 (dAsCpf1-SRDX)

   Plasmid

   #86209

   Purpose

   Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   dAsCpf1
   

   Use

   Gateway compatible cpf1 vectors

   Tags

   NLS and SRDX repressor and NLS

   Expression

   Plant

   Mutation

   D908A

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_052_HygroR

   Plasmid

   #228937

   Purpose

   Plasmid for cloning of custom gRNA using EnAsCas12a

   Depositor

   Francisca Vazquez

   Article

   Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

   Plasmid

   #199605

   Purpose

   Expression of cell permeable opAsCas12a in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   opCas12a-T8N
   

   Use

   CRISPR

   Tags

   6xHis, Twin-strep, SUMO

   Expression

   Bacterial

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. WN10150

   Plasmid

   #80443

   Purpose

   Expresses dead (908A) AsCpf1

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   nuclease dead (908A) AsCpf1
   

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   nuclease dead (D908A)

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA052H_sgCh2

    Plasmid

    #229023

    Purpose

    Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA052H_sgFOCAD #2

    Plasmid

    #229024

    Purpose

    Expression of a EnAsCas12a guide targeting FOCAD

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    FOCAD gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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