We narrowed to 11,440 results for: Kars

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pcDNA3.1(+)-sapphireAKAR(T/A)

Plasmid

#118465

Purpose

Negative-control mutant for sapphireAKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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MyrPalm-CKAR-T/A

Plasmid

#14863

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

T/A

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
MyrPalm-BKAR

Plasmid

#14878

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
negEKARet-cyto

Plasmid

#114734

Purpose

Negative control of EKARet-cyto

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

Negative Control of EKARet-cyto

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-blueCKAR(T/A)

Plasmid

#118473

Purpose

Negative-control mutant for blueCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pRSET B-CKAR

Plasmid

#14868

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

6xHis/Xpress, CFP, and YFP

Expression

Bacterial

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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EKARet-PSD

Plasmid

#114723

Purpose

PSD-targeted EKARet

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

PSD-targeted EKARet

Tags

EGFP and N-terminus of PSD-95

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW AKAR3-EV-NR

Plasmid

#182534

Purpose

Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

Depositor

Bas Teusink

Article

Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

Insert

AKAR3-EV-NR

Expression

Yeast

Mutation

T506A mutation

Promoter

PMA1

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET B-BKAR

Plasmid

#14877

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP and YFP

Expression

Bacterial

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pSBbi-Puro_LPAR_AKAR-WT

Plasmid

#125206

Purpose

SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter

Depositor

Eric Davis

Insert

LPAR_AKAR-WT

Use

Transposon

Tags

EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET B-DKAR

Plasmid

#14881

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2007 Mar 2. 282(9):6733-42.

Insert

D kinase activity reporter

Tags

CFP and YFP

Expression

Bacterial

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-rsAKARev

Plasmid

#166231

Purpose

Expresses reversibly switchable A-kinase activity reporter with EV-linker (rsAKARev) in mammalian cells

Depositor

Peter Dedecker

Article

Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

Insert

Reversibly switchable A-kinase activity reporter with EV-linker

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS_m-AKAR5’

Plasmid

#114140

Purpose

Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5' C-terminally tagged by KRas C tail

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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EKAREN5-CFPonly

Plasmid

#246759

Purpose

Control plasmid for EKAREN5 without YPet cassette (expressing CFP only)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-CFPonly

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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EKAREN5-YFPonly

Plasmid

#246760

Purpose

Control plasmid for EKAREN5 without mTurquioise2 cassette (expressing YFP only)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-YFPonly

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mBJH-AKAR3EV

Plasmid

#236206

Purpose

Fluorescent biosensor for cytosolic PKA activity

Depositor

Peter Dedecker

Article

Van Thillo et al ACS Nano. 2025 Jul 1;19(25):22755-22766.

Insert

QC2-AKAR-Halotag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mSGH-AKAR3EV

Plasmid

#236207

Purpose

Fluorescent biosensor for cytosolic PKA activity

Depositor

Peter Dedecker

Article

Van Thillo et al ACS Nano. 2025 Jul 1;19(25):22755-22766.

Insert

QC2-AKAR-Halotag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-deltaCKAR

Plasmid

#222429

Purpose

FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Plasma membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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CCKAR-DuET

Plasmid

#213195

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCKAR (CCKAR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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