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Flag‐mTim1 (Bl/6)

Plasmid

#49205

Purpose

expresses Flag tagged mouse Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (BL/6 allele) (Havcr1 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
STIMATE(1-240)

Plasmid

#71280

Purpose

Expresses STIMATE or TMEM110 (1-240) in mammalian cells

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

STIMATE (1-240) (TMEM110 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
Stim1-CaRQ

Plasmid

#34560

Depositor

Kevin Truong

Article

Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Insert

Stim1-CaRQ (STIM1 Human)

Use

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L in RhoA

Promoter

CMV, T7

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
Flag‐Tim2

Plasmid

#49213

Purpose

expresses Flag tagged Tim2

Depositor

Lawrence Kane

Article

Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

Insert

Tim2 (Timd2 Mouse)

Use

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

no Tim2 signal sequence; R80L and S196D compared …

Promoter

EF1a

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only
pLAS2w.Phyg-TIMD4

Plasmid

#232670

Purpose

expresses TIMD4 in mammalian cells

Depositor

Chen-Yang Shen

Insert

TIMD4 (TIMD4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-TIMP1

Plasmid

#186979

Purpose

Expresses mouse TIMP1 in mammalian cells

Depositor

Yong-Rui Zou

Article

Song et al J Exp Med. 2023 Dec 4;220(12):e20230018.

Insert

tissue inhibitor of metalloproteinase 1 (Timp1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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STIM1-2Strep

Plasmid

#210361

Purpose

Expresses STIM1 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

STIM1 (STIM1 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-HisTim12_CtoA

Plasmid

#170281

Purpose

Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

Insert

yeast Tim12 (TIM12 Budding Yeast)

Use

Tags

TEV-cleavable N-terminal His tag (on backbone)

Expression

Bacterial

Mutation

mutated two non-conserved Cys to Ala

Promoter

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-optimizedNedd4.2F823D

Plasmid

#165105

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d F823D

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

Insert

Nedd4.2d F823D (NEDD4L Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression; F823D; …

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFS_0367_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1

Plasmid

#116979

Purpose

Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Woodsholea maritima RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0368_pET30-RT-CRISPR_Woodsholea_maritima_ARRAY_1_RC

Plasmid

#116980

Purpose

Expresses WmRT-Cas1-Cas2 under pT7lac promoter, encodes WmCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Woodsholea maritima RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-MitoTimer

Plasmid

#50547

Purpose

Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

Depositor

Roberta Gottlieb

Article

Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

Insert

MitoTimer

Use

Tags

Expression

Mammalian

Mutation

F12S mutation in Timer

Promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Flag-Timeless

Plasmid

#22887

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

Insert

Timeless (TIMELESS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted normal start codon, replaced with start c…

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-Optimized UBE1L

Plasmid

#165099

Purpose

ecoli optimized expression of GST-fusion of human Ube1L

Depositor

Arthur Haas

Insert

UbeE1L (UBA7 Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry-Stim1

Plasmid

#186620

Purpose

Third generation lentiviral vector expressing Stim1-wt fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hTimeless (pc4729)

Plasmid

#216861

Purpose

Mammalian expression vector encoding GFP tagged human Timeless

Depositor

Cristina Cardoso

Article

Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

Insert

Timeless (TIMELESS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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MultiMate-Cell-cycle

Plasmid

#206280

Purpose

Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

H2B iRFP713, mAG-hGeminin, mKO2-hCdt1

Use

Recombinant baculovirus production

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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human STIM1 CFP

Plasmid

#19755

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Use

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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