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Showing: 141 - 160 of 1419 results

  2. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid
    #117134
    Purpose
    All-in-one expression vector for Cas9 and gRNA against CD44
    Depositor
    Kosuke Yusa
    Insert
    gCD44 v2 (Cd44 Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, human EF1a promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117134-plasmid-map-sequence-id-226805

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  3. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  4. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid
    #201917
    Purpose
    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201917-plasmid-map-sequence-id-403313

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  5. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  6. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

    Plasmid
    #194925
    Purpose
    mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho
    Depositor
    Hao Xu
    Article
    Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    hPGK
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194925-plasmid-map-sequence-id-393400

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  10. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid
    #159086
    Purpose
    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.
    Depositor
    Jeremy Day
    Article
    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589
    Insert
    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    EGFP and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    hSYN, U6
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159086-plasmid-map-sequence-id-310691

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  15. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

    Plasmid
    #129530
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme
    Depositor
    Erik Sontheimer
    Article
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Expression
    Mutation
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    129530-plasmid-map-sequence-id-261485

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  17. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)

    Plasmid
    #117139
    Purpose
    Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP
    Depositor
    Kosuke Yusa
    Article
    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste
    Insert
    HA-Dmap1 (mutagenised) (Dmap1 Synthetic)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    modified BFP, modified Dmap1 CDS
    Promoter
    human U6 promoter, human EF1a promoter
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    117139-plasmid-map-sequence-id-243795

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  19. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  20. pMuLE ENTR U6-miR-30 L1-R5

    Plasmid
    #62113
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62113-plasmid-map-sequence-id-101402

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Showing: 141 - 160 of 1419 results