We narrowed to 922 results for: cas12a

1. pHS638_Flex-Cas12a-CBE

   Plasmid

   #244845

   Purpose

   Mammalian expression of Flex-Cas12a cytosine base editor

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Flex-dCas12a-CBE
   

   Use

   CRISPR

   Tags

   2xSV40 NLS-APOBEC3A and Nucleoplasmid NLS-UGI-SV4…

   Expression

   Mammalian

   Mutation

   G146R/R182V/D535G/S551F/D665N/E795Q/D832A

   Promoter

   CMV

   Available since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

   Plasmid

   #244836

   Purpose

   Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   2xSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEM4_CL7_Bac_2NLS-WT (LbCas12a)-4NLS

   Plasmid

   #244839

   Purpose

   Bacterial expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS641_Flex-Cas12a-ABE

   Plasmid

   #244846

   Purpose

   Mammalian expression of Flex-Cas12a adenine base editor

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Flex-dCas12a-ABE
   

   Use

   CRISPR

   Tags

   SV40 NLS-ABE8e

   Expression

   Mammalian

   Mutation

   G146R/R182V/D535G/S551F/D665N/E795Q/D832A

   Promoter

   CMV

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS

   Plasmid

   #244843

   Purpose

   Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Lbm6-Cas12a
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 4xSV40 NLS

   Expression

   Bacterial

   Mutation

   D535G/S551F/D665N

   Promoter

   T7

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQdCas12a.luxR(mut)-sggfp(B)

   Plasmid

   #236041

   Purpose

   The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQdCas12a.luxR(mut)-sggfp(A)

   Plasmid

   #236039

   Purpose

   The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAST-uMCas12a+nls2x

   Plasmid

   #230909

   Purpose

   inducible expression of Cas12a+ with Gal4.

   Depositor

   Michael Boutros

   Article

   Port et al bioRxiv 2024.11.26.625385

   Insert

   Cas12a+
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-LbCas12a-8xNLS

   Plasmid

   #209020

   Purpose

   Lentiviral vector expressing human codon-optimized LbCas12a with 8xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   LbCas12a-8xNLS-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas12a-AID

    Plasmid

    #193647

    Purpose

    Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hAID, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-dCas12a-CDA1

    Plasmid

    #193649

    Purpose

    Expression of base editor dCas12a-CDA1 in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-PmCDA1, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-dCas12a-eAID

    Plasmid

    #193650

    Purpose

    Expression of base editor dCas12a-eAID in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-eAID, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 6xHis-MBP-huMlCas12a

    Plasmid

    #174678

    Purpose

    Expresses 6xHis-MBP tagged MlCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMlCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid

    #176261

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid

    #176245

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid

    #176246

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 6xHis-MBP-huCMaCas12a

    Plasmid

    #174679

    Purpose

    Expresses 6xHis-MBP tagged CMaCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMaCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6xHis-MBP-huPxCas12a

    Plasmid

    #174675

    Purpose

    Expresses 6xHis-MBP tagged PxCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPxCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6xHis-MBP-huPrCas12a

    Plasmid

    #174674

    Purpose

    Expresses 6xHis-MBP tagged PrCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPrCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6xHis-MBP-huBfCas12a

    Plasmid

    #174672

    Purpose

    Expresses 6xHis-MBP tagged BfCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBfCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge