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Addgene
Showing: 141 - 160 of 2310 results
  1. AAVS1-idCas9-vpr

    Plasmid
    #89985
    Purpose
    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus
    Depositor
    Insert
    dCas9-VPR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid
    #80768
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pV1093

    Plasmid
    #111428
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
    Depositor
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pMZ288

    Plasmid
    #59897
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+
    Depositor
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pMZ289

    Plasmid
    #59898
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210
    Depositor
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pYZ675

    Plasmid
    #132954
    Purpose
    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe
    Depositor
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pYZ694

    Plasmid
    #132956
    Purpose
    crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe
    Depositor
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pMZ381

    Plasmid
    #59899
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469
    Depositor
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 141 - 160 of 2310 results