We narrowed to 24,415 results for: lars;

1. pET21-pelB-LaRIgG-2

   Plasmid

   #228996

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-2
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaRIgG-7

   Plasmid

   #228997

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaRIgG-1

   Plasmid

   #228995

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

   Plasmid

   #228508

   Purpose

   Bacterial Hybrid Driver Plasmid

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   T7 RNAP, LARP-I5CHO (#1)
   

   Use

   Synthetic Biology

   Tags

   Umu Degron, GTG-start codon

   Expression

   Mutation

   R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFlare9A-Clip2E9 Mutant

   Plasmid

   #209575

   Purpose

   minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Clip2 (Clip2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

   Promoter

   CMV

   Available since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Modular BE4max for GG insertion

   Plasmid

   #198891

   Purpose

   Easily insert deaminase of choice into BE4max using Golden Gate cloning

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   CRISPR associated protein 9 (cas9 Streptococcus pyogenes)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-DmLARK_N

   Plasmid

   #146981

   Purpose

   Insect Expression of DmLARK

   Depositor

   Elisa Izaurralde

   Insert

   DmLARK (lark Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1B-lambdaN-HA-DmLarp_N

   Plasmid

   #146982

   Purpose

   Insect Expression of DmLarp

   Depositor

   Elisa Izaurralde

   Insert

   DmLarp (larp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two non silent mutation N110T, P173L compared to …

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFS_0387_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2

   Plasmid

   #116999

   Purpose

   Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Rivularia sp. PCC 7116 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFS_0388_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_2_RC

    Plasmid

    #117000

    Purpose

    Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 2 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Rivularia sp. PCC 7116 RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_0386_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1_RC

    Plasmid

    #116998

    Purpose

    Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Rivularia sp. PCC 7116 RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Kohinoor-Fibrilarin/pcDNA3

    Plasmid

    #67781

    Purpose

    Fibrilarin-targetted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor-Fibrilarin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

13. Phamret-Fibrillarin/pcDNA3

    Plasmid

    #51954

    Purpose

    mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to Fibrillarin for nucleolar localization

    Depositor

    Takeharu Nagai

    Article

    Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

    Insert

    Phamret
    

    Use

    Tags

    Fibrillarin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSP2-48-merTetO-EFS-BLaR

    Plasmid

    #118712

    Purpose

    Contains an optimized 48-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 LARP7-FLAG 7SK

    Plasmid

    #200733

    Purpose

    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK

    Depositor

    Claudio Alarcón

    Insert

    LARP7 and 7SK (LARP7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. psfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164477

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Lari-BFP2-TSERex

    Plasmid

    #123311

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Lari-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PR_GEF (2XPDZ-mCherry-Larg (DH)

    Plasmid

    #80407

    Purpose

    Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

    Depositor

    Michael Glotzer

    Article

    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

    Insert

    2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    DH domain only (aa 766-997)

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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