We narrowed to 936 results for: multisite gateway

Showing: 141 - 160 of 936 results

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pME Actin-Vhh sfGFP (JDW 1215)

Plasmid

#224495

Purpose

Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet3-Giantin (JDW 1356)

Plasmid

#224502

Purpose

Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-Giantin

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mRuby2-3xMyc (JDW 1246)

Plasmid

#242554

Purpose

Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mRuby2

Use

Gateway Cloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mBaoJin-WPRE (JDW 1383)

Plasmid

#242549

Purpose

Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mBaoJin

Use

Gateway Cloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1508)

Plasmid

#242555

Purpose

Gateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway Cloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-EF1a-pA (JDW 1222)

Plasmid

#224526

Purpose

A Gateway compatible 3' entry clone containing rat EF-1a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF-1a polyA

Use

Gateway Cloning

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pME AmCyan (JDW 1151)

Plasmid

#224485

Purpose

Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

AmCyan

Use

Gateway Cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mCherry-3xHA (JDW 1321)

Plasmid

#224493

Purpose

Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mCherry-3xHA

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1367)

Plasmid

#224503

Purpose

Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myc-BirA (JDW 1210)

Plasmid

#224509

Purpose

Gateway compatible middle entry clone containing a Myc tagged BirA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BirA

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS1 (JDW 1095)

Plasmid

#224510

Purpose

Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BioID2

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS2 (JDW 1209)

Plasmid

#224511

Purpose

A Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCS

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BioID2

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mClover3-pA (JDW 1318)

Plasmid

#224530

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mClover3-pA

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAM-kan-R4R3

Plasmid

#71275

Purpose

Binary destination vector for plant transformation. Kanamycin resistance.

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Type

Empty backbone

Use

Gateway Cloning

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-2xFYVE_hrs

Plasmid

#67676

Purpose

Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2xFYVEhrs

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-SpCas9-HF1-2A-EGFP-L2

Plasmid

#141048

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF1-2A-EGFP

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-Cas9n-EGFP-L2

Plasmid

#141046

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9n-2A-EGFP

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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