We narrowed to 480 results for: rtta

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piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

Plasmid

#209077

Purpose

Ngn2 mediated cortical neuron induction, dox induction is included

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
SNAPtag

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

Plasmid

#69546

Purpose

Tet-On system expressing MyoD-2A-GFP-2xNLS.

Depositor

Charles Gersbach

Inserts

eGFP
Myod1 (Myod1 Mouse)

Use

Lentiviral

Tags

2x nuclear localization sequence and T2A "sk…

Mutation

Removed stop codon to fuse it to the 2A-eGFPnls i…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

Plasmid

#209082

Purpose

NFIB and SOX9 mediated astrocye induction

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
mKate2

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

Plasmid

#177203

Purpose

encoding stblHIF under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)

Plasmid

#85800

Purpose

donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro

Plasmid

#61441

Purpose

Dox-inducible MyoD with mRNA tag for smFISH

Depositor

Charles Gersbach

Article

Gibson et al Integr Biol (Camb). 2015 Jun 8;7(6):663-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#138149

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS-HA

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#223173

Purpose

Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hfCas13d (N2V8)

Use

Lentiviral

Tags

NLS-HA

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

Plasmid

#209078

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

3K mutant: E35K, E46K, E61K

Promoter

TRE4G

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

Plasmid

#140229

Purpose

Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

AntiCRISPR protein AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

Plasmid

#223174

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

Plasmid

#239974

Purpose

PiggyBac plasmid for inducible PEmax expression and Puromycin selection

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA-IRES-nls-EGFP

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

Plasmid

#229839

Purpose

A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

Plasmid

#242578

Purpose

PiggyBac transposon flanked, Tet-on, gateway destination vector.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#228558

Purpose

Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

Insert

dhfCas13d (N2V8)

Use

Lentiviral

Tags

NLS-HA

Mutation

R239A/H244A/R858A/H863A

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

Plasmid

#105842

Purpose

Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
Optimized mApple
rtTA3G
SBP Delta-LNGFR

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

Plasmid

#221234

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Use

Lentiviral

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

Plasmid

#209080

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

A53T

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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