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Plasmid#209043PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)DepositorInsertnSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-opCas12a-Ultra-6xNLS
Plasmid#209024PurposeLentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLSDepositorInsertopCas12a-Ultra-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/M537R/S542R/F870LPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0030:Dendra
Plasmid#159040PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0030
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_5048:Dendra
Plasmid#167366PurposeC-term fluorescent fusionDepositorInsertMSMEG_5048
UseIntegrating mycobacterial expression vector attl5…TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0028:Dendra
Plasmid#159038PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0028
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0132:Dendra
Plasmid#159002PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0132
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0319:Dendra
Plasmid#159010PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0319
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0311:Dendra
Plasmid#159052PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0311
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_1346:Dendra
Plasmid#174997PurposeC-term fluorescent fusionDepositorInsertMSMEG_1346
UseIntegrating mycobacterial expression vector attl5…TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0709:Dendra
Plasmid#159077PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0709
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-∆ex8
Plasmid#209050PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)DepositorInsertTAF5 deltaexon-8
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-miniTurbo-TAF5-∆ex8
Plasmid#209052PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to TAF5 isoform lacking the alternative exon-8 (∆ex8)DepositorInsertTAF5 deltaexon-8
UseTagsminiTurboExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-FL_WobbleMut
Plasmid#209055PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNADepositorInsertTAF5 full length
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut
Plasmid#209056PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNADepositorInsertTAF5 deltaexon-8
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-NoCBE
Plasmid#209038PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)DepositorInsertnSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-APOBEC1
Plasmid#209039PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLSDepositorInsertAPOBEC1-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-BEACON2
Plasmid#209041PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLSDepositorInsertBEACON2-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-evoCDA1
Plasmid#209042PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLSDepositorInsertevoCDA1-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-TadA8.20m
Plasmid#209045PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLSDepositorInsertTadA8.20m-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET01-TAF5-ex8-Minigene-SRSF1 motif MUT
Plasmid#218973PurposeVector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)DepositorInsertTAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
UseTagsExpressionMammalianMutationmutant SRSF1 binding motif (AGTCTCCT)PromoterRSV LTRAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only