We narrowed to 2,368 results for: pcas9

1. pBECKP-km

   Plasmid

   #117235

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_Hygro

   Plasmid

   #167859

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2T-CMV-EA-BE4max-NG-BlastR

   Plasmid

   #152992

   Purpose

   High purity C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4 H47E+S48A-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-TJP1

   Plasmid

   #227299

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Eef1a-1955/-1>nls::Cas9::nls

   Plasmid

   #59987

   Purpose

   Eef1a (EF1alpha) promoter driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Eef1a (EF1alpha) -1955/-1

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScI_dCas9-CDA-UL

   Plasmid

   #108551

   Purpose

   Bacterial Target-AID UGI-LVA vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1-UGI-LVA
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sox1/2/3-2373/+3>nls::Cas9::nls

   Plasmid

   #59989

   Purpose

   Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Sox1/2/3 (SoxB1) -2373/+3

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Expression

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. ePPE-V223A

   Plasmid

   #205243

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   SV40_NLS-nCas9-NC-MLV-SV40_NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. NAX_CAGGS_CAS9_Blasto

    Plasmid

    #167856

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRU319

    Plasmid

    #167686

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. XLone-Cas9-2A-GFP

    Plasmid

    #170159

    Purpose

    Express Cas9 and GFP upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    Cas9
    

    Use

    Synthetic Biology; Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G promoter

    Available Since

    July 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS415_pGal-nCas9(D10A)

    Plasmid

    #79618

    Purpose

    Expresses nCas9(D10A) in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-CMV-AIDmax-BlastR

    Plasmid

    #152994

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    AID-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-CMV-ABEmaxCP1041-BlastR

    Plasmid

    #152990

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9 CP1041
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2T-CMV-BE4maxCP1028-BlastR

    Plasmid

    #152993

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    BE4-nSpCas9 CP1028-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p2T-CMV-CDAmax-BlastR

    Plasmid

    #152995

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    CDA-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH-A3A-PBE

    Plasmid

    #119774

    Purpose

    Conversion of C to T in rice plants transformation

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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