We narrowed to 7,241 results for: 11

1. pcDNA3.1 3xF-GAR1

   Plasmid

   #126873

   Purpose

   Expresses 3xFlag GAR1

   Depositor

   Kathleen Collins

   Article

   Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

   Insert

   GAR1 (GAR1 Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-GFP-NMHC II-A

   Plasmid

   #10844

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLoxP-DHFR-mCherry

   Plasmid

   #70147

   Purpose

   Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

   Depositor

   David Sibley

   Article

   Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

   Insert

   a selectable dihydrofolate reductase-thymidylate synthase marker
   

   Use

   Expression in toxoplasma gondii

   Tags

   mCherry

   Promoter

   DHFR 5' UTR

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rtn2b-GFP

   Plasmid

   #186599

   Purpose

   Encodes human full-length Reticulon 2 isoform B fused to GFP

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B (RTN2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIntegrase_9

   Plasmid

   #60580

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_9
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gq sensor

   Plasmid

   #137810

   Purpose

   A FRET sensor for Gq activation, encoded on a single plasmid

   Depositor

   Dorus Gadella

   Article

   Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

   Insert

   Gq sensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRB_LD 0_CS G_tTA-BFP

   Plasmid

   #58876

   Purpose

   MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-PIK3R1

   Plasmid

   #174192

   Purpose

   Expresses PIK3R1 in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gata3-T2A-H2B-eGFP donor

   Plasmid

   #113119

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Gata3 donor region including homology arms and T2A-H2B-eGFP
   

   Use

   Synthetic Biology

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fgfr2-T2A-H2B-Halo donor

    Plasmid

    #113117

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Fgfr2 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTEV5_ RpMatB_1

    Plasmid

    #78205

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

    Insert

    matB (His6 C106A R286C Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A R286C Q457C K488A

    Promoter

    T7

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101104

    Purpose

    Encodes dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-LRF

    Plasmid

    #66936

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV-40

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 Plk4(Sak) D154A (Nigg HR26)

    Plasmid

    #41164

    Depositor

    Erich Nigg

    Article

    Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

    Insert

    Plk4 D154A (PLK4 Human)
    

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    D154A

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

    Plasmid

    #51690

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    hisG-URA3-hisG (URA3 Budding Yeast)
    

    Use

    Yeast marker swap

    Expression

    Yeast

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbB8k-csg-amylase

    Plasmid

    #166859

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mVenus-PA-PKI

    Plasmid

    #65456

    Purpose

    photoactivatable PKA inhibition

    Depositor

    Klaus Hahn

    Article

    Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

    Insert

    PKI (PKIA, Pkia Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

18. plvx-AD8

    Plasmid

    #191558

    Purpose

    Inducible expression of HIV-1 Env

    Depositor

    Alon Herschhorn, Joseph Sodroski

    Article

    Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

    Insert

    HIV-1 AD8 env
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EcMBP165-cpGFP.PPYF.T203V.pRSET

    Plasmid

    #33373

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    Improved MBP based Maltose Biosensor
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available Since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. GEV 2

    Plasmid

    #12616

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)
    

    Tags

    G protein and His

    Expression

    Bacterial

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only