Skip to main content
Addgene
Showing: 1581 - 1600 of 6852 results

  10. pMSP1E1D1

    Plasmid
    #161605
    Purpose
    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Ritchie et al Methods Enzymol. 2009 . 464():211-31.
    Insert
    MSP1E1D1
    Use
    Tags
    7-His
    Expression
    Bacterial
    Mutation
    "extended" MSP1D1; contains repeat of h…
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161605-plasmid-map-sequence-id-317650

    Enlarge

  12. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

    Enlarge

  14. pTEV5_ RpMatB_1

    Plasmid
    #78205
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7
    Insert
    matB (His6 C106A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pMSP1E2D1

    Plasmid
    #161606
    Purpose
    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Ritchie et al Methods Enzymol. 2009 . 464():211-31.
    Insert
    MSP1E2D1
    Use
    Tags
    7-His
    Expression
    Bacterial
    Mutation
    "extended" MSP1D1; contains repeat of h…
    Promoter
    T7
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161606-plasmid-map-sequence-id-318420

    Enlarge

Showing: 1581 - 1600 of 6852 results