We narrowed to 1,873 results for: FRT
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Plasmid#175065Purposeexpresses mVenus and mCherry tagged DNAJB6b in mammalian cellsDepositorInsertDNAJB6b (DNAJB6 Human)
UseTagsV5 and mVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-MBP-TEV
Plasmid#105771PurposeExpression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsMBP-TEVExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mClover3
Plasmid#105795PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mClover3ExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e
Plasmid#90010PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3eDepositorInsertsCBirA*-Cdc25C WT
NBirA*-14-3-3e
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mVenus
Plasmid#105791PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mVenusExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GDNF-SHC
Plasmid#139624PurposeStrepIII-HA-tagged gene expressionDepositorAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5 TEX264wt-cSSH
Plasmid#220209PurposeMammalian expression of wild type TEX264, C-terminal twin-Strep_HA tagDepositorInsertTEX264 (TEX264 Human)
UseTagsStrep-strep-HAExpressionMammalianMutationPromoterCMV/TetOAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-BirA-FLAG
Plasmid#105786PurposeExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-BirA-FLAGExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-MBP
Plasmid#105787PurposeExpression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-MBPExpressionMammalianMutationPromoterAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-3XFLAG
Plasmid#105784PurposeExpression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-3XFLAGExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GRB2-SHC
Plasmid#139623PurposeStrepIII-HA-tagged gene expressionDepositorAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-mEGFP-TEV
Plasmid#105777PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).DepositorTypeEmpty backboneUseFlp-in competentTagsmEGFP-TEVExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
5'-3xFLAG Chtop-exon minimal CMV pCDNA5
Plasmid#212080PurposeLR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cellsDepositorAvailable SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKK-EGFP-TEV
Plasmid#105776PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsEGFP-TEVExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-HA-TEV
Plasmid#105769PurposeExpression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsHA-TEVExpressionMammalianMutationPromoterAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-HA
Plasmid#105785PurposeExpression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-HAExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mTagBFP-TEV
Plasmid#105772PurposeExpression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsmTagBFP-TEVExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-NoTag
Plasmid#105767PurposeControl vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.DepositorTypeEmpty backboneUseFlp-in competentTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-LAP-KNL1-M3
Plasmid#115896PurposeUsed for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertKNL1-M3 (KNL1 Human)
UseTagsEYFPExpressionMammalianMutationFragment of KNL1 containing three KNL1 repeat seq…PromoterAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF4A2-C106R
Plasmid#31123DepositorInsertHNF4A (HNF4A Human)
UseTagsMycExpressionMammalianMutationsplice variant 2, mutation C106R. Wild type seq…PromoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only