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AP568-2

Plasmid

#70047

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pGEL080

Plasmid

#137889

Purpose

iSTU-CRISPR/Cas9 with OsCDPK2 intron, NU structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL085

Plasmid

#137893

Purpose

iSTU-CRISPR/Cas9 with RcCat intron, tRNA structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL083

Plasmid

#137892

Purpose

iSTU-CRISPR/Cas9 with RcCat intron, NU structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ675

Plasmid

#132954

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

nucleoplasmin NLS

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ694

Plasmid

#132956

Purpose

crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA4

Plasmid

#174034

Purpose

In vitro transcription of anti-CRISPR AcrIIA4 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA4

Use

In vitro transcription

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA2

Plasmid

#174033

Purpose

In vitro transcription of anti-CRISPR AcrIIA2 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA2

Use

In vitro transcription

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crF1

Plasmid

#213046

Purpose

Guide RNA expression vector with the synthetic crRNA crF1 targeting the SWITCHER construct with the target site TF1

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crF1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crR2

Plasmid

#213047

Purpose

Guide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crR2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b_TypeIC_acrIC4_invivo

Plasmid

#196403

Purpose

expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IC4 protein

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC3

Plasmid

#174036

Purpose

In vitro transcription of anti-CRISPR AcrIIC3 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC3

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC1

Plasmid

#174035

Purpose

In vitro transcription of anti-CRISPR AcrIIC1 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC1

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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JME5000

Plasmid

#129661

Purpose

EYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL086

Plasmid

#137894

Purpose

iSTU-CRISPR/Cas9 with RcCat intron, RZ structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL077

Plasmid

#137886

Purpose

iSTU-CRISPR/Cas9 with StIV2 intron, NU structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL079

Plasmid

#137888

Purpose

iSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL078

Plasmid

#137887

Purpose

iSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Blast

Plasmid

#52962

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Has Service

Lentiviral Prep

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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