Addgene

We narrowed to 13,554 results for: TIM;

Showing: 1581 - 1600 of 13554 results

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pEB2-2ndVal mKate2

Plasmid

#104030

Purpose

Plasmid encoding 2ndVal mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mKate2

Use

Low copy

Tags

Expression

Bacterial

Mutation

mKate2 with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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4xmEGFP

Plasmid

#127801

Purpose

Homo-tetramer mEGFP-mEGFP-mEGFP-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mEGFP-mEGFP-mEGFP-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFH13

Plasmid

#125798

Purpose

Level0 SpCas9, wheat codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SpCas9, wheat codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-mEYFP

Plasmid

#103985

Purpose

Plasmid encoding mEYFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEYFP

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, V68L, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFH17

Plasmid

#125803

Purpose

Level0 LbCas12a, rice codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, rice codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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TroponinT-Gateway

Plasmid

#46026

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-VSV-G-HiBiT

Plasmid

#162594

Purpose

C-terminally HiBiT-tagged VSV-G under CMV promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

VSV-G

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet1

Plasmid

#107444

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met1

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-TurboRFP

Plasmid

#103996

Purpose

Plasmid encoding TurboRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TurboRFP

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, T68A, L79F, …

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

Plasmid

#169326

Purpose

Expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEB1-mEGFP

Plasmid

#103979

Purpose

Plasmid encoding mEGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEGFP

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS-CyRFP1

Plasmid

#84546

Purpose

Bacterial expression of CyRFP1

Depositor

Michael Lin

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-6XDSR

Plasmid

#48980

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-4XDSR

Plasmid

#48978

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCVII-AM

Plasmid

#162751

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

ametrine

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+FFlucWT

Plasmid

#26158

Depositor

Siouxsie Wiles, Brian Robertson

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Firefly luciferase

Use

Tags

Expression

Bacterial

Mutation

Lacks the last 3 aa.

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pDZ207 (loxP KAN loxP 24xPP7SL MDN1)

Plasmid

#35191

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

loxP KAN loxP 24xPP7SL MDN1

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only

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DinoIII-arrO-N

Plasmid

#128143

Purpose

Dinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marina

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

Rifampin ADP-ribosylating transferase + potential promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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2xmScarlet-I

Plasmid

#127807

Purpose

Homo-dimer mScarlet-I-mScarlet-I expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mScarlet-I-mScarlet-I

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YSOG2

Plasmid

#42227

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Use

Tags

TS:YSOG2

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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