164,826 results

1. pET28a-gp32-H6

   Plasmid

   #163912

   Purpose

   Expresses T4 gp32 for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   gp32
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus2-Y705F/S727A-STAT3

   Plasmid

   #123177

   Purpose

   Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   Double Y705F and S727A substitutions

   Promoter

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_426

   Plasmid

   #179097

   Purpose

   BE Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   ABE8e
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3/Myc-DNMT3B1

   Plasmid

   #35522

   Purpose

   Mammalian expression of human DNMT3B1 with myc tag

   Depositor

   Arthur Riggs

   Article

   Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

   Insert

   DNMT3B1 (DNMT3B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEntr_Myc-BirA-G3-ER

   Plasmid

   #173814

   Purpose

   Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

   Depositor

   Norbert Perrimon

   Article

   Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

   Insert

   Myc-BirA-G3-ER
   

   Use

   Gateway shuttle vector

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCXIP-turboRFP

   Plasmid

   #73016

   Purpose

   Retroviral expression of turboRFP

   Depositor

   Michael Grusch

   Insert

   TurboRFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEYFP-N3-EPAC1

   Plasmid

   #113110

   Purpose

   Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

   Depositor

   Xiaodong Cheng

   Article

   Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

   Insert

   RapGEF3 (RAPGEF3 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   PCMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. plRL19

   Plasmid

   #163634

   Purpose

   L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Insert

   L5 Integrase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Mycobacterial Optimized Promoter (MOP) and Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0282

    Plasmid

    #141561

    Purpose

    Lentiviral vector for overexpressing the MEF2C transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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