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We narrowed to 6,626 results for: GFP expression plasmids

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Showing: 4001 - 4050 of 6626 results

  8. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

    Plasmid
    #223112
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223112-plasmid-map-sequence-id-447635

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  9. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid
    #223115
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223115-plasmid-map-sequence-id-447640

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  10. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid
    #223116
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223116-plasmid-map-sequence-id-447639

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  15. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215011
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215011-plasmid-map-sequence-id-429274

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  16. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid
    #197505
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…
    Promoter
    CMV and T7
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197505-plasmid-map-sequence-id-386783

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  17. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid
    #197506
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197506-plasmid-map-sequence-id-386645

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  18. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]

    Plasmid
    #124069
    Purpose
    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
    Use
    CRISPR and Cre/Lox
    Expression
    Insect
    Promoter
    bhsp68
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124069-plasmid-map-sequence-id-240664

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  19. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121800
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121800-plasmid-map-sequence-id-237308

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  21. pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

    Plasmid
    #92225
    Purpose
    Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    deGFP Y35TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue Y35 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92225-plasmid-map-sequence-id-197052

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  22. YIplac211-VPS54-msGFP2x3

    Plasmid
    #255068
    Purpose
    Insert a C-terminal msGFP2x3 tag at VPS54 by popin/popout.
    Depositor
    Benjamin Glick
    Insert
    Vps54-msGFP2x3
    Expression
    Yeast
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    255068-plasmid-map-sequence-id-512489

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  23. YIplac211-msGFP2-IMH1

    Plasmid
    #255059
    Purpose
    Insert an N-terminally tagged msGFP2-Imh1 sequence at the IMH1 locus. This is a second copy integration.
    Depositor
    Benjamin Glick
    Insert
    msGFP2-Imh1
    Expression
    Yeast
    Available Since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    255059-plasmid-map-sequence-id-511747

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  25. ER-EGFP-FLAG-APEX2

    Plasmid
    #251714
    Purpose
    Expresses ER localized EGFP-FLAG-APEX2 in mammalian cells
    Depositor
    Svetlana Konovalova
    Insert
    ER signal peptide (APEX2 Human)
    Tags
    APEX2, EGFP, and FLAG
    Expression
    Mammalian
    Available Since
    April 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    251714-plasmid-map-sequence-id-504834

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  28. His-13bCTS-GFP

    Plasmid
    #247243
    Purpose
    A bacterial expression plasmid encoding ciliary targeting sequence of mammalian Arl13b (amino acids 347-363) with N-terminal His and GFP-tagged
    Depositor
    Lei Lu
    Article
    Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi
    Insert
    Arl13B (ARL13B Human)
    Tags
    6xHis and EGFP
    Expression
    Bacterial
    Mutation
    deleted amino acid 1-346,364-428
    Promoter
    T7 Promoter
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    247243-plasmid-map-sequence-id-496173

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  29. YIplac211-Gga2-msGFPx3

    Plasmid
    #247735
    Purpose
    Insert a C-terminal msGFP2x3 tag at GGA2 by popin-popout
    Depositor
    Benjamin Glick
    Insert
    Gga2-msGFP2x3
    Expression
    Yeast
    Available Since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    247735-plasmid-map-sequence-id-494804

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  30. YIplac211-msGFP2-Pmr1

    Plasmid
    #247742
    Purpose
    Insert an N-terminal msGFP2 tag at PMR1 by popin-popout
    Depositor
    Benjamin Glick
    Insert
    msGFP2-Pmr1
    Expression
    Yeast
    Available Since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    247742-plasmid-map-sequence-id-492066

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  31. YIplac211-Ric1-msGFP2x4

    Plasmid
    #247746
    Purpose
    Insert a C-terminal msGFP2x4 tag at RIC1 by popin-popout
    Depositor
    Benjamin Glick
    Insert
    Ric1-msGFP2x4
    Expression
    Yeast
    Available Since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    247746-plasmid-map-sequence-id-492080

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  32. YIplac211-msGFP2-Vrg4

    Plasmid
    #247743
    Purpose
    Insert an N-terminal msGFP2 tag at VRG4 by popin-popout
    Depositor
    Benjamin Glick
    Insert
    msGFP2-Vrg4
    Expression
    Yeast
    Available Since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    247743-plasmid-map-sequence-id-492079

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  33. YIplac211-Kre2-moxGFP2

    Plasmid
    #247741
    Purpose
    Insert a C-terminal moxGFP2 tag at KRE2 by popin-popout
    Depositor
    Benjamin Glick
    Insert
    Kre2-moxGFP2
    Expression
    Yeast
    Available Since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    247741-plasmid-map-sequence-id-492071

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  34. YIplac211-Sys1-msGFP2x3

    Plasmid
    #247752
    Purpose
    Insert a C-terminal msGFP2x3 tag at SYS1 by popin-popout
    Depositor
    Benjamin Glick
    Insert
    Sys1-msGFP2x3
    Expression
    Yeast
    Available Since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    247752-plasmid-map-sequence-id-492051

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  35. YIplac211-Gnt1-moxGFP2

    Plasmid
    #247737
    Purpose
    Insert a C-terminal moxGFP2 at GNT1 by popin-popout
    Depositor
    Benjamin Glick
    Insert
    Gnt1-moxGFP2
    Expression
    Yeast
    Available Since
    Nov. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    247737-plasmid-map-sequence-id-492045

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  36. TRPML1 Y499A-EGFP

    Plasmid
    #245040
    Purpose
    Expresses TRPML1 mutant with C-terminus EGFP tag in mammalian cells
    Depositor
    Thomas Martin
    Insert
    TRPML1 (MCOLN1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Y499A
    Available Since
    Oct. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    245040-plasmid-map-sequence-id-488276

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  38. EGFP Rab3a F59S

    Plasmid
    #245020
    Purpose
    Effector binding rat Rab3a F59S mutant
    Depositor
    Thomas Martin
    Insert
    Rab3a (Rab3a Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245020-plasmid-map-sequence-id-488292

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  39. EGFP Rab3a IDF/A3

    Plasmid
    #245016
    Purpose
    Effector binding mutant of rat Rab3a
    Depositor
    Thomas Martin
    Insert
    Rab3a (Rab3a Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245016-plasmid-map-sequence-id-487718

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  40. GFP Rab3 GAP1

    Plasmid
    #245050
    Purpose
    Distribution and localization of human wild-type GAP1 in cells
    Depositor
    Thomas Martin
    Insert
    GAP1
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245050-plasmid-map-sequence-id-487701

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  44. pFA6a-psfGFP-kanMX6

    Plasmid
    #240551
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with superfolder GFP, codon-optimized for S. pombe
    Depositor
    Silke Hauf
    Insert
    psfGFP
    Expression
    Yeast
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240551-plasmid-map-sequence-id-479473

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  45. pFA6a-psfGFP-hphNT1

    Plasmid
    #240552
    Purpose
    Tag S. pombe genes at 3' end (C-terminal end of protein) with superfolder GFP, codon-optimized for S. pombe
    Depositor
    Silke Hauf
    Insert
    psfGFP
    Expression
    Yeast
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    240552-plasmid-map-sequence-id-479466

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  47. Grx1-roGFP2-PSPC1

    Plasmid
    #236549
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in paraspeckles.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Grx1-roGFP2-PSPC1 (PSPC1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    236549-plasmid-map-sequence-id-475413

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Showing: 4001 - 4050 of 6626 results