We narrowed to 2,521 results for: crispr system

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EC0_6_sgRNA

Plasmid

#163717

Purpose

Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

sgRNA expression cassette

Use

Synthetic Biology; Moclo vector level 0 (type 234)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

Plasmid

#87906

Purpose

sgRNA targeting GFP

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgGFP2, sgGFP3

Expression

Mammalian

Promoter

H1, U6, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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LLP339 pGK-dCas9-SunTag

Plasmid

#100943

Purpose

dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-B-Seipin-miniIAA7-mEGFP

Plasmid

#129719

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mEGFP

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry-weak NLS

Plasmid

#129717

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry-weak NLS

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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gfap-EGFP donor

Plasmid

#65564

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap-EGFP (gfap Zebrafish)

Use

Zebrafish injection

Promoter

none

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-B-AtAFB2-mCherry

Plasmid

#129718

Purpose

AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

Plasmid

#129722

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-3XFlag

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I

Plasmid

#129721

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mScarlet-I

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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LLP457 pGK-dCas9-Suntag-BFP

Plasmid

#100957

Purpose

BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA and BFP

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-Seipin-miniIAA7-mCherry

Plasmid

#129720

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-mCherry

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDIV313

Plasmid

#204956

Purpose

AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
hph (hygromycin resistance marker)
sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus fumigatus U3 promoter, Aspergillus ni…

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA-10Xcapture-PuroR

Plasmid

#211496

Purpose

Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

Depositor

Antonella Fidanza, Lesley Forrester

Article

Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

U6-gRNA
Ef1a-PuroR

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

EF1a and U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

Plasmid

#177345

Purpose

AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

dead hSaCas9

Use

AAV and CRISPR

Tags

3x GCN4 peptide, 3xHA, and NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

Synapsin

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pNS19-LV-mutRFP-2A-GFP

Plasmid

#113716

Purpose

Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

mutRFP-2A-GFP-IRES-Puro

Use

Lentiviral; Reporter system

Expression

Mammalian

Mutation

Y64L (mutRFP)

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHS1862

Plasmid

#205975

Purpose

FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1859

Plasmid

#205972

Purpose

FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Csy3/Cas7 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1860

Plasmid

#205973

Purpose

FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6/Csy4 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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