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pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo

Plasmid

#62346

Purpose

Expresses rtTA2sM2 and tTSkid in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

rtTA2sM2-IRES-tTSkid

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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yWL268 MCP-GFP

Plasmid

#197052

Purpose

pUBC-NLS-HA-2xMCP-2xGFP

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-2xGFP

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pRSetB-Peredox

Plasmid

#73806

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Tags

his-tag to facilitate purification

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-Halo-tDeg

Plasmid

#191370

Purpose

Overexpression of Halo-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

Halo-tDeg

Tags

Halo

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

Plasmid

#205161

Purpose

express β-ACTIN-3′UTR-24xMS2

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

β-ACTIN-3′UTR-24xMS2

Tags

tagBFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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mKate-pBAD

Plasmid

#54826

Purpose

Localization: Protein Expression Vector , Excitation: 588, Emission: 635

Depositor

Dmitriy Chudakov, Michael Davidson

Article

Shcherbo et al Nat Methods. 2007 Sep . 4(9):741-6.

Type

Empty backbone

Tags

6xHis and mKate

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pUC19-TgKI-MCS-tdTomato-polyA

Plasmid

#174022

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

tdTomato

Use

Zebrafish knock-in tagging

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-Hygro

Plasmid

#184593

Purpose

EGFP

Depositor

Robert Sobol

Article

Koczor et al Methods Mol Biol. 2023;2609:43-59. doi:

Insert

EGFP

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H2_beta_CM

Plasmid

#184870

Purpose

pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

March 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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paavCAG-pre-mGRASP-2A-dTomato

Plasmid

#51902

Purpose

pre-2Ato

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRetroSuper Smad3

Plasmid

#15726

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad3 shRNA (SMAD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_HAIB

Plasmid

#184869

Purpose

For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_H2_beta_GM

Plasmid

#184871

Purpose

pSwap plasmid part with H2_beta homology arms and gentamycin cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mCyRFP1-C1

Plasmid

#84355

Purpose

Expresses mCyRFP1 fluorescent protein in an empty backbone

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1

Expression

Mammalian

Mutation

A161K and N41A, reletive to CyRFP1

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDQM045

Plasmid

#247633

Purpose

to generate mRNA for membrane mScarlet3 for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mScarlet3

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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B-gTEMP/pRSET-b

Plasmid

#188446

Purpose

B-gTEMP for bacterial expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Tags

6xHis-tag

Expression

Bacterial

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ GCamp6-3NLS

Plasmid

#165401

Purpose

Expresses a nuclear localized genetically encoded calcium reporter.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

GCamp6

Expression

Mammalian

Promoter

SP6

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 1

Plasmid

#134343

Purpose

Mammalian acoustic reporter gene 1 cassette 1 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 1

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 2

Plasmid

#134344

Purpose

Mammalian acoustic reporter gene 1 cassette 2 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-3xGFP-tDeg

Plasmid

#191371

Purpose

Overexpression of 3xGFP-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

3xGFP-tDeg

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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