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We narrowed to 2,616 results for: pCas

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Showing: 1601 - 1620 of 2616 results

  2. sgKRAS(B)

    Plasmid
    #85567
    Purpose
    Expresses sgRNA targeting human KRAS around codon G12
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAS proto-oncogene, GTPase (KRAS Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  3. sgKRAS(A)

    Plasmid
    #85566
    Purpose
    Expresses sgRNA targeting human KRAS around codon G12
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAS proto-oncogene, GTPase (KRAS Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  4. px330 VRER

    Plasmid
    #101729
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VRER
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101729-plasmid-map-sequence-id-265859

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  6. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #129418
    Purpose
    Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 and sgRAB18.N (RAB18 Human)
    Use
    CRISPR
    Tags
    T2A-mCherry-P2A-Puro
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129418-plasmid-map-sequence-id-254820

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  7. pMIA3-Pansio

    Plasmid
    #214445
    Purpose
    Expresses eSpCas9 and gRNA targeting Pansio-1 GSH
    Depositor
    Roger Foo
    Publication
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-P2A-mRuby2-P2A-tp53dn
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    214445-plasmid-map-sequence-id-433843

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  8. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135014-plasmid-map-sequence-id-267599

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  11. PDG458 V3

    Plasmid
    #226959
    Purpose
    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226959-plasmid-map-sequence-id-450819

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  12. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid
    #140004
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140004-plasmid-map-sequence-id-275398

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  13. EF1a-TET1-dCas9

    Plasmid
    #235593
    Purpose
    Tet1CD fused to N-terminus of dCas9
    Depositor
    Ting Zhou
    Publication
    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
    Use
    CRISPR
    Tags
    3xNLS, TET1 CD, and tagBFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    235593-plasmid-map-sequence-id-467826

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  15. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73196-plasmid-map-sequence-id-134659

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  17. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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  18. pMCRi

    Plasmid
    #207524
    Purpose
    Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Publication
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Use
    Pseudomonas vector
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207524-plasmid-map-sequence-id-409593

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  20. PB-PEmax SpG

    Plasmid
    #187895
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, SpG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187895-plasmid-map-sequence-id-370756

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Showing: 1601 - 1620 of 2616 results