We narrowed to 1,667 results for: plasmid for e coli

1. pET26b_Nb.b201

   Plasmid

   #131404

   Purpose

   E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody b201
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSETa-mMaple3-V157I

   Plasmid

   #98577

   Purpose

   photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mMaple3-V157I
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET26b_Nb.AD101

   Plasmid

   #131402

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody AD101
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMW96

   Plasmid

   #178066

   Purpose

   Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

   Insert

   CDK5RAP2 (CDK5RAP2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GB1-ALIX(P801G)

   Plasmid

   #180025

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   P801G

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-optimizedNedd4.2d

   Plasmid

   #165103

   Purpose

   bacterial expression of a GST fusion of Human Nedd4.2d

   Depositor

   Arthur Haas

   Article

   Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

   Insert

   Nedd4.2d (NEDD4L Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T-1-Optimized E6AP

   Plasmid

   #165100

   Purpose

   ecoli optimized expression of GST-fusion of human E6AP

   Depositor

   Arthur Haas

   Article

   Ronchi et al J Biol Chem. 2013 Apr 12;288(15):10349-6

   Insert

   E6AP isoform 3 (UBE3A Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLyGo-Ec-7

   Plasmid

   #163135

   Purpose

   pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Nanobody, C-IgAP autotransporter

   Expression

   Bacterial

   Mutation

   Promoter

   P(rhaB)

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCosSAD1AprR

   Plasmid

   #190786

   Purpose

   An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

   Depositor

   Jan Kormanec

   Article

   Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

   Inserts

   upstream region of the aur1 cluster for auricin
   ampramycin resistance cassette
   downstream region of the aur1 cluster for auricin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

    Plasmid

    #191647

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

    Plasmid

    #191635

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSETa-mEos3.2-A69T

    Plasmid

    #98571

    Purpose

    photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mEos3.2-A69T
    

    Use

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET26b_Nb.AD102

    Plasmid

    #131403

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody AD102
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSETa-mKikGR-V69T

    Plasmid

    #98574

    Purpose

    photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mKikGR-V69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLei-sfGFP-V2*D134*Y152*

    Plasmid

    #191112

    Purpose

    An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    superfolder green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changing V2 & D134 & Y152 to TAG

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GB1-PRD(703-868, P801G)

    Plasmid

    #180029

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJL1-MBP-DQNAT

    Plasmid

    #198215

    Purpose

    pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    MBP_DQNAT
    

    Use

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)

    Plasmid

    #191646

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

    Plasmid

    #160540

    Purpose

    Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

    Inserts

    SARS-CoV-2 NSP7 (ORF1ab Synthetic)
    SARS-CoV-2 NSP8 (ORF1ab Synthetic)
    SARS-CoV-2 NSP12 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pExp-RBD-CHis

    Plasmid

    #195002

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Article

    Fischer et al bioRxiv 2023.01.10.523494

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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