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We narrowed to 5,364 results for: puc

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Showing: 1601 - 1620 of 5364 results

  1. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

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  3. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid
    #115161
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.
    Inserts
    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    Use
    AAV
    Tags
    hM3Dq-mCherry
    Expression
    Mammalian
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115161-plasmid-map-sequence-id-220939

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  8. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid
    #99694
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    99694-plasmid-map-sequence-id-194587

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  13. pSCW01

    Plasmid
    #72300
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72300-plasmid-map-sequence-id-255196

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  15. pSCW02

    Plasmid
    #72301
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72301-plasmid-map-sequence-id-255198

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Showing: 1601 - 1620 of 5364 results