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pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Article

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8s

Plasmid

#169256

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO mScarlet

Plasmid

#131002

Purpose

Double floxed mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

mScarlet

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Chronos-GFP

Plasmid

#99232

Purpose

AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2G

Plasmid

#159605

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2G

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Has Service

AAV1, AAV2, and AAV9

Insert

NIR-GECO2G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Puro

Plasmid

#227266

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Human, Synthetic)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2

Plasmid

#159603

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

NIR-GECO2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS1-hChR2-tBFP

Plasmid

#178706

Purpose

AAV vector for transgene expression of hChR2-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

hChR2-tBFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLY088_AAV_EFS-BCMA-41BBz-PRODH2

Plasmid

#192191

Purpose

BCMA CAR AAV vector PRODH2 KI (pLY088)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

BCMA CAR AAV vector PRODH2 KI (pLY088) (TNFRSF17 Synthetic)

Use

AAV; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT3.0mut

Plasmid

#208718

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8f

Plasmid

#192615

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_5-HT1.0

Plasmid

#140552

Purpose

Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

Depositor

Yulong Li

Article

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Has Service

AAV9

Insert

5-HT sensor GRAB_5-HT1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1 hPGK-PuroR-pA donor

Plasmid

#22072

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PGK-Puro (PGK1 Human)

Use

Zfn donor

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-jGCaMP8m-WPRE

Plasmid

#179255

Purpose

AAV-mediated expression of GCaMP8m under the CAG promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-tdTomato-WPRE

Plasmid

#51506

Purpose

Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV5

Insert

tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

phSyn1

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95-mCherry

Plasmid

#125694

Purpose

Exogenous expression of PSD95 (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CKIIa-stGtACR1-FusionRed

Plasmid

#105679

Purpose

Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Insert

stGtACR1

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-EGFP-KASH

Plasmid

#209201

Purpose

Neuronal expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Insert

eGFP-KASH

Use

AAV

Tags

KASH localization domain

Expression

Mammalian

Mutation

Promoter

hsyn

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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