We narrowed to 3,519 results for: Braf;
-
-
p3E-EHD1a-dEH
Plasmid#109565PurposeMultisite gateway vector for 3' tagging with EHD1a-delta-EHDepositorInsertEHD1a (zgc:77049 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdelta-EHPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
kank1a_pJet1.2
Plasmid#121978Purposefor generating kank1a riboprobes for WISH in zebrafishDepositorInsertkank1a (si:dkey-146l2.1 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mdm1_pJet1.2
Plasmid#121977Purposefor generating mdm1 riboprobes for WISH in zebrafishDepositorInsertmdm1 (mdm1 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
1132 pME-Lin28
Plasmid#49009Purposefull length coding sequence for zebrafish lin28 gene in gateway middle entry vectorDepositorInsertlin-28 homolog A (lin28ab Zebrafish)
UseGateway middle entryTagsExpressionMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
666 pME-Lnx-1
Plasmid#49006Purposefull length coding sequence for zebrafish lnx1 gene in gateway middle entry vectorDepositorInsertligand of numb-protein X 2b (lnx2b Zebrafish)
UseGateway middle entryTagsExpressionMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
p075 pCS xTRPS1 delN delC119 (M)
Plasmid#11045DepositorInsertTRPS1 delN delC119 mut (trps1 Frog)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationaa806-1153 of xTRPS1. Two Cys residues in the GA…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p076 pCS xTRPS1 delN delC320 (M)
Plasmid#11046DepositorInsertTRPS1 (trps1 Frog)
UseMammalian, avian, and zebrafishTagsExpressionMutationaa806-952 of xTRPS1. Two Cys residues in the GAT…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p063 pCS xTRPS1 delta N (M)
Plasmid#11036DepositorInsertTRPS1 deltaN mut (trps1 Frog)
UseMammalian, avian, and zebrafishTagsExpressionMutationaa806-1153 of xTRPS1. Two Cys residues in the GA…PromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Rab11a (Q70L)
Plasmid#184037PurposeExpresses constitutively active mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
UseTagsmCherryExpressionMammalianMutationGlutamine 70 changed to LeucinePromoterCMV IE94Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-Rac1-DN
Plasmid#109582PurposeMultisite gateway vector for 3' tagging with dominant negative Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pEGFP-N1-human lyn–GFP
Plasmid#35958PurposeExpresses human lyn protein codon optimized for zebrafish expression.DepositorInsertLYN (LYN Human)
UseTagsEGFPExpressionMammalianMutationwtPromotercmvAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME-MLS-KillerRed
Plasmid#115517PurposeMiddle entry plasmid containing membrane bound KillerRed fluorophoreDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST1374-NLS-flag-linker-Cas9
Plasmid#44758DepositorInsertHuman codon optimized Cas9
UseCRISPRTags32 amino acid linker, Flag, and NLSExpressionMammalianMutationcodon-optimizedPromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Rab11a (S25N)
Plasmid#184036PurposeExpresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
UseTagsmCherryExpressionMammalianMutationSerine 25 changed to AsparaginePromoterCMV IE94Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
neurod1:Gal4; cmlc2:EGFP
Plasmid#173478PurposeGal4 is driven by -5kb neurod1 promoter. The vector contains a cmlc2:EGFP markerDepositorInsertGal4
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-0.349cldn15la
Plasmid#125026PurposeFive prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.DepositorInsertClaudin 15-like a (cldn15la Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-KDEL-pA
Plasmid#109546PurposeMultisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)DepositorInsertmKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol1:uas-myc-act-map2k2b-2a-tdTomato
Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagExpressionMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5E-pth2
Plasmid#191428PurposeTol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)DepositorInsertpth2 regulatory region (pth2 Zebrafish)
UseTol2 gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-PLK1-mCherry
Plasmid#127154PurposeExpresses mCherry PLK1 in mammalian cells and zebrafish embryosDepositorInsertPLK1-mCherry (PLK1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-RhoAb-DN
Plasmid#109580PurposeMultisite gateway vector for 3' tagging with dominant negative RhoAbDepositorInsertRhoAb (rhoab Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ macroH2A1.2-GFP-HA
Plasmid#30515DepositorInsertmacroH2A1.2-C (Macroh2a1 Mouse)
UseXenopus/zebrafish expression.TagseGFP and triple HAExpressionMammalianMutationNO STOPPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E-ING2
Plasmid#109554PurposeMultisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsert3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Akt3 WT-2X Flag
Plasmid#87607Purposeexpresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 proteinDepositorInsertAkt3 isoform 2 (AKT3 Human)
UseZebrafish (danio rerio) expressionTags2 tandem flag tags and His-Halo-TevExpressionMammalianMutationAkt3 isoform 2PromoterSP6Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag Smad3 EPSM V179T
Plasmid#26998DepositorInsertSmad3 (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationT179V S204A S208A S213A; V179TPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pME DN-E-cadherin
Plasmid#135209PurposeDominate Negative zebrafish e-cadherin labelDepositorInsertCDH1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
645 pCS2-etv2
Plasmid#49005PurposeMammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2DepositorInsertets variant gene 2 (etsrp Zebrafish)
UseInvitro transcription, riboprobe templateTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad2 (100-467)
Plasmid#14928DepositorInsertSmad2 (100-467) (SMAD2 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationamino acids 100-467PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad2 (1-185)
Plasmid#14929DepositorInsertSmad2 (1-185) (SMAD2 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationamino acids 1-185PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
459 pME-etv2
Plasmid#49002Purposefull length coding sequence for zebrafish etv2 gene in gateway middle entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway middle entryTagsExpressionMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3E-FAPP1
Plasmid#109555PurposeMultisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4PDepositorInsertthe PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
kank2_pJet1.2
Plasmid#121980Purposefor generating kank2 riboprobes for WISH in zebrafishDepositorInsertkank2 (LOC571549 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag Smad3 EPSM A208S
Plasmid#27112DepositorInsertSmad3 (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationT179V S204A S208A S213A; A208SPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag Smad3 EPSM A213S
Plasmid#27113DepositorInsertSmad3 (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationT179V S204A S208A S213A; A213SPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
Tol2-UAS-Dendra2-miR-223 sponge
Plasmid#97146PurposeFor miR-223 knock downDepositorInsertmiR-223 sponge
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only
