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pKOR1-mcs

Plasmid

#181755

Purpose

Allele replacement for Staphylococcus aureus

Depositor

Suzan Rooijakkers

Article

Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMM131

Plasmid

#127214

Purpose

BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, AtSTM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-STAT3-linker-TagGFP2

Plasmid

#121427

Purpose

WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

STAT3-linker-TagGFP2

Tags

TagGFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-EZH2

Plasmid

#222528

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

EZH2 (EZH2 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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miR34a in pcDNA3.1

Plasmid

#78125

Purpose

Mammalian expression of miR34

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR34 (MIR34A Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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raACR-mCerulean3-pAAV2-hSyn

Plasmid

#204966

Purpose

Panneuronal expression of the MsACR1 mutant raACR fused to mCerulean3

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Use

AAV

Tags

mCerulean3

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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BFP Homology Donor

Plasmid

#149372

Purpose

Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Insert

CDDR BFP Homology Donor

Expression

Mammalian

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pZZMP26

Plasmid

#231755

Purpose

Mammalian expression of TOP6BL

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

TOP6BL (Gm960 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH MERIT40

Plasmid

#27498

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

MERIT40 (BABAM1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pmiRFP703-Myosin

Plasmid

#79995

Purpose

LC-Myosin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

LC-Myosin (Myl9 Mouse)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH KIAA0157

Plasmid

#27499

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

KIAA0157 (ABRAXAS2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only
eGFP L202 gRNA

Plasmid

#119132

Purpose

gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L202 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45564

Purpose

Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

d14 eGFP donor
I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Mutation

deleted n' 15 amino acids

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO-shSmad4

Plasmid

#37046

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad4 (SMAD4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR2b-PTPN12

Plasmid

#215477

Purpose

Gateway entry vector with PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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MsACR1-mCerulean3-pAAV2-hSyn

Plasmid

#204965

Purpose

Panneuronal expression of MsACR1 fused to mCerulean3

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

MsACR1

Use

AAV

Tags

mCerulean3

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRG706_lexO-SbfI_LexA-TF_LEU2MX

Plasmid

#154818

Purpose

Inducible expression of SbfI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

SbfI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PM

Plasmid

#197386

Purpose

To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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human MTAP

Plasmid

#64077

Purpose

Expression of human 5'-methylthioadenosine phosphorylase

Depositor

Vern Schramm

Article

Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

Insert

5'-methylthioadenosine phosphorylase (MTAP Human, Synthetic)

Tags

Histidine tag and TEV

Expression

Bacterial

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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