174,125 results
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Plasmid#160735PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVS1-VIR2
Plasmid#134745PurposeVirulence and replication helperDepositorInsertvir gene cluster
UseVir gene expressionAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xHA-TurboID pRetrox
Plasmid#155203PurposeFor doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait proteinDepositorInsert3xHA-TurboID
UseRetroviralTags3xHAExpressionMammalianPromotertight TRE promotorAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp5_Mpro
Plasmid#156475PurposeBacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tagDepositorInsertNsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX(exo-)-6xHis
Plasmid#145028PurposepET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tagDepositorArticleInsertRTX(exo-)
Tags6xHisExpressionBacterialMutationN210DPromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV
Plasmid#140135PurposeCan be used to generate AAV virus that will express light-inducible site-specific iCreV recombinaseDepositorHas ServiceAAV PHP.eBInsertiCreV
UseAAV and Cre/LoxExpressionMammalianPromoterEF1alphaAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.N
Plasmid#127851Purposenon-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.N VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NFIA+SOX9
Plasmid#129455PurposeInducible overexpression of NFIA and Sox9 in human cells.DepositorAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_ACh3.0
Plasmid#121923Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent mannerDepositorInsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVPromoterhSynAvailable SinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6XHis-human SUMO 1
Plasmid#133770Purposeexpress 6Xhis- SUMO 1 proteinDepositorAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only