164,577 results

1. mCherry-Vimentin-7

   Plasmid

   #55156

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pDD120

   Plasmid

   #119879

   Purpose

   Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pConstitutive_Che9c60 and Che9c61
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX307-TMPRSS2-G418

   Plasmid

   #158459

   Purpose

   Constitutive lentiviral expression of human TMPRSS2.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pExp-Sumo

   Plasmid

   #112574

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8His and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLumi-LgBiT

   Plasmid

   #188319

   Purpose

   Expression of LgBiT via sigma 70 promoter in E. coli

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   LgBiT
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sigma 70 promoter

   Available Since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1(+)-NEMOs

   Plasmid

   #189931

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-2NLS-Cas9-6XMS2

   Plasmid

   #166033

   Purpose

   Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

   Plasmid

   #107941

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a (E174R/S542R/K548R)
   

   Use

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R and K548R

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-TGNP-N-10

   Plasmid

   #55145

   Purpose

   Localization: Golgi Complex, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   TGNP (TGOLN2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiRFP713-N1

    Plasmid

    #136559

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP713
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge