161,838 results

1. pDONR221_SLC1A2

   Plasmid

   #131901

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC1A2 (SLC1A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKP864

   Plasmid

   #167658

   Purpose

   Express colicinB-His6

   Depositor

   Kathleen Postle

   Insert

   cba-his6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   cba-his6

   Promoter

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-FLAG-GFP-OSBP

   Plasmid

   #134659

   Purpose

   Flag and GFP-tagged OSBP

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   OSBP (OSBP Human)
   

   Use

   Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIB4

   Plasmid

   #25453

   Depositor

   Benjamin Glick

   Article

   Sears et al Yeast. 1998 Jun 15;14(8):783-90.

   Insert

   Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
   

   Use

   Pichia pastoris expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-PalmGRET

   Plasmid

   #158221

   Purpose

   BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

   Depositor

   Charles P. Lai

   Article

   Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

   Insert

   PalmGFP-Nluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNHEJ-recX-sacB

   Plasmid

   #158718

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pmyc

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pJAT17

   Plasmid

   #204298

   Purpose

   For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-EYFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCG_SARS-CoV1 C-V5-tag ORF3a

    Plasmid

    #179969

    Purpose

    Mammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 ORF3a (ORF3a Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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