We narrowed to 2,346 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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1. pCDH-EF1a-tetR-T2A-iRFP

   Plasmid

   #176028

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   iRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 RL3Sc

   Plasmid

   #109365

   Purpose

   Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDECKO_Malat1_B

   Plasmid

   #72621

   Purpose

   Expresses two gRNAs targeting MALAT1 promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward Malat1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-LbCas12a-Plus-mCherry

   Plasmid

   #191230

   Purpose

   To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   LbCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R, R883K, R887A

   Promoter

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-CAG-NCreIntN-EF1a-mTagBFP2

   Plasmid

   #160507

   Purpose

   Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRW006-SM-destination-pro35S-Cas9

   Plasmid

   #104439

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   35S

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQTEV-PRKRA

   Plasmid

   #31571

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA

   Plasmid

   #97010

   Purpose

   Rosa26 targeting vector for FLP-responsive cassette

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC267

   Plasmid

   #118887

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6-MBP, cleavable with 3C protease; HALO tag

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP97

    Plasmid

    #83551

    Purpose

    Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CYC1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPMVAugw

    Plasmid

    #98300

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TEFM1EfmvaE>TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PliaI

    Plasmid

    #55177

    Purpose

    Bacitracin-inducible promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    liaI promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    liaI

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-HA-dSpCas9

    Plasmid

    #92112

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9
    

    Use

    CRISPR

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ230-RV

    Plasmid

    #188544

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V

    Promoter

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOCC167

    Plasmid

    #118909

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PliaG

    Plasmid

    #55174

    Purpose

    Constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    LiaG promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCVpic2blast-EGFP-FF2

    Plasmid

    #164923

    Purpose

    Expresses EGFP cDNA and FF2 shRNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

    Inserts

    Enhanced green fluorescent protein
    Neuropeptide FF receptor 2 (NPFFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-hSyn-IntCCCre-T2A-CopGFP

    Plasmid

    #160503

    Purpose

    Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    March 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ETV7_pet28a

    Plasmid

    #131656

    Purpose

    Bacterial expression of ETS gene ETV7

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV7 (ETV7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAYC

    Plasmid

    #62657

    Purpose

    Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge