We narrowed to 14,061 results for: CAN
-
Plasmid#220448PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
ExpressionInsectAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(1)
Plasmid#220447PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
ExpressionInsectAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_gfp
Plasmid#212136PurposeCargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_CbFDH
Plasmid#212140PurposeCargo plasmid for integrating PT7 expression cassette of formate dehydrogenase in genome. Plasmid can be removed by incubating cells at 37 C.DepositorInsertformate dehydrogenase
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-sgHPRT1
Plasmid#196713PurposeCRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TGDepositorInsertCas9-T2A-BSD-U6-sgHPRT1
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1a/hU6Available SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj12a
Plasmid#173141PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12a (kcnj12a Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj16
Plasmid#173146PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj16a (kcnj16 Zebrafish)
UsePcr cloning vectorMutationStop codon removedPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-wtBlc
Plasmid#168257PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondinglyDepositorInsertC-fragment of wtBlc
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
shCDK4(1)
Plasmid#73550PurposeshRNA(1) against CDK4DepositorInsertshRNA against CDK4
UseRetroviralExpressionMammalianPromoterLTRAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
shCDK4(2)
Plasmid#73551PurposeshRNA(2) against CDK4DepositorInsertshRNA against CDK4
UseRetroviralExpressionMammalianPromoterLTRAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-ZNF668
Plasmid#45042DepositorInsertZNF668 (ZNF668 Human)
ExpressionBacterialAvailable SinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-N1
Plasmid#60561PurposeExpresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteinsDepositorInsertmTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBA-mCherry-EGFP-PIM
Plasmid#111758PurposeLow level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
ExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …Promoterchicken beta-actinAvailable SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-LbNOX
Plasmid#75285PurposeThis plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertLbNOX
TagsC-terminal FLAG tag with linkerMutationHuman codon optimizedAvailable SinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lck-mScarlet-I
Plasmid#98821PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorInsertLck
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
TagsFlag tag with linker and mitochondrial targeting …MutationHuman codon optimizedAvailable SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only