We narrowed to 2,325 results for: dcas9

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KU801: pMVP (L5-L4) mCherry

Plasmid

#121709

Purpose

pMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KU901: pMVP (L5-L4) eCFP

Plasmid

#121711

Purpose

pMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KV001: pMVP (L5-L4) eYFP

Plasmid

#121713

Purpose

pMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

Plasmid

#119070

Purpose

Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled1-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled2-sgRNA-Ds

Plasmid

#119071

Purpose

Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled2-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

Plasmid

#119072

Purpose

Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled3-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

Plasmid

#119073

Purpose

Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled4-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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JP705: pMVP/SB/Neo-DEST

Plasmid

#121866

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_120

Plasmid

#96917

Purpose

for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

VPR

Expression

Mammalian

Mutation

D10A, N863A

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK614

Plasmid

#110608

Purpose

BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

dCas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PB-SAM

Plasmid

#102559

Purpose

Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Inserts

MS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Mutation

D10A and N863A in Cas9 and N55K in MS2

Promoter

CAG and EF1A

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

Plasmid

#84258

Purpose

Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgTRE3G
PYL1-VPR

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

Plasmid

#84260

Purpose

Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgSV40
PYL1-KRAB

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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KP201: pMVP (L5-L4) eGFP-P2A

Plasmid

#121708

Purpose

pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ173

Plasmid

#99907

Purpose

Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Insert

pco-dCas9-VP64-T2A-MS2-VP64

Use

CRISPR

Expression

Plant

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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LLP777_SnoopCatcher-p65HSF1-CO

Plasmid

#211770

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SnoopCatcher-p65HSF1

Tags

2xOLLAS

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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KP501: pMVP (L5-L4) eYFP-P2A

Plasmid

#121714

Purpose

pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Biomolecular FeedBack (FB)

Plasmid

#109391

Purpose

This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pJ23100mut-dCas9-T- phtpG1-sgRNA

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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