We narrowed to 5,838 results for: dna plasmids

1. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176237

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176238

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SV.signed

   Plasmid

   #133933

   Purpose

   To validate the use of digital signatures in DNA.

   Depositor

   Jean Peccoud

   Insert

   lacZ and digital signature cassette
   

   Expression

   Bacterial

   Available Since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMis1_1B

   Plasmid

   #192079

   Purpose

   A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

   Depositor

   Markus Buchhaupt

   Article

   Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

   Inserts

   KanR
   rhaR
   rhaS
   pBBR1 rep
   

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

   Plasmid

   #203803

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Q68L

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

   Plasmid

   #203802

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   T23N

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH131

   Plasmid

   #48260

   Purpose

   Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

   Inserts

   URA3 3' fragment
   URA3 5' fragment
   GAL10/GAL1 bidirectional promoter
   

   Use

   Routine cloning vector

   Mutation

   -242 upstream to URA3 ORF +495 and URA3 ORF from …

   Promoter

   GAL10/GAL1 bidirectional

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-EAMP

   Plasmid

   #138265

   Purpose

   mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   iCas9 target-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1a-HTLV

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBluescript II NLS-MC-EGFP-tdTomato (pBET2)

   Plasmid

   #240411

   Purpose

   This plasmid facilitates the assessment of MMR proficiency in human living cells

   Depositor

   Isao Kuraoka

   Article

   Takedachi et al Mutat Res. 2022 Jan-Jun;824:111779. doi:

   Insert

   NLS-EGFP fusion protein
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB1C3-FeGFP

    Plasmid

    #138261

    Purpose

    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    eGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    None

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172318

    Purpose

    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22B_SA_PcrA

    Plasmid

    #102999

    Purpose

    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker

    Depositor

    Martin Webb

    Insert

    SA_PcrA
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    PcrA 199-2423

    Promoter

    T7

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4 [luc2P/ERE/Hygro] Vector

    Plasmid

    #236826

    Purpose

    Express ERE response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ERE
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGL4 [luc2P/3A4/Hygro] Vector

    Plasmid

    #236827

    Purpose

    Express 3A4 response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    3A4
    

    Use

    Luciferase

    Mutation

    None

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGL4 [luc2P/2B6/Hygro] Vector

    Plasmid

    #236828

    Purpose

    Express 2B6 response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    2B6
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pNL [NlucP/ARE/Hygro] Vector

    Plasmid

    #236818

    Purpose

    Express ARE response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ARE
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pTER_MDC1 (422-2)

    Plasmid

    #22191

    Depositor

    Eric Campeau

    Insert

    sh Mediator of DNA damage checkpoint 1
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Jan. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRV-TdTom-MCS

    Plasmid

    #193711

    Purpose

    Retroviral plasmid DNA for subcloning an oligonucleotide library encoding RNA barcodes

    Depositor

    Colin Akerman

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC-ODN

    Plasmid

    #208642

    Purpose

    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Long single strand dna production

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-CMV-scFVDnmt3AL_bGHpA

    Plasmid

    #177347

    Purpose

    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    C-terminal domain of mouse Dnmt3l (194–415 aa), P…

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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