161,911 results

1. pT7-7 asyn A53T

   Plasmid

   #105727

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A53T (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A53T

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-spCas9 ABE-Umax-ex2-rest1

   Plasmid

   #222143

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3TS-spCas9 ABE-Umax-ex1-rest1

   Plasmid

   #222140

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2258

   Plasmid

   #142927

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX11 (SOX11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQP-T2A

   Plasmid

   #102583

   Purpose

   Light-controlled activation of expression

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

   Insert

   BphP1-VP16-T2A-NLS-GAL4-QPAS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH2B-LSSmOrange

   Plasmid

   #37133

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

   Insert

   LSSmOrange
   

   Use

   Tags

   histone 2B

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available since

   June 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-Synaptojanin 1-170

   Plasmid

   #22292

   Depositor

   Pietro De Camilli

   Article

   Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

   Insert

   Synaptojanin 1_170 (SYNJ1 Human)
   

   Use

   Tags

   Flag

   Expression

   Bacterial and Mammalian

   Mutation

   K334R compared to Genbank ID NM_003895

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. CASR-Tango

   Plasmid

   #66235

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CASR (CASR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule-pCNF

    Plasmid

    #85494

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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